Articles
Germination responses of Dyera costulata seeds following cryopreservation
Article number
1234_10
Pages
79 – 84
Language
English
Abstract
The possibility of seed cryopreservation of Dyera costulata, an important commercial timber species, was studied.
Matured pods were collected from a tree stand in grounds of the Forest Research Institute Malaysia.
Upon receipt at the laboratory, the seeds were removed manually from the pods.
Fresh seeds were immediately subjected to liquid nitrogen storage and another set of control seeds were kept 20°C cool room for up to six weeks.
Seed samples were withdrawn after 1, 2, 4, and 6 weeks.
Prior to storage and after each storage period, moisture content (MC) and germination of the seeds were checked.
Fresh seeds had 8.3% MC and 93.3% germinated.
Seeds kept under liquid nitrogen temperature (-196°C) had c. 6% MC. However, germination was reduced from 90 to 50% throughout the storage time.
Meanwhile, control seeds showed an increased in MC during the storage, from 7.1 to 8.3%. Germination of the control seeds fell from 90% after one week in storage to 65% after six weeks.
These findings showed that D. costulata seeds tolerated dry storage at both cool (20°C) and ultra-cold (-196°C) temperature for up to six weeks, although the germination percentage decreased under both conditions.
Surviving germinated seeds were transferred to vermiculite for growth evaluation.
Further studies are needed to determine the most suitable seed MC for D. costulata cryopreservation.
Matured pods were collected from a tree stand in grounds of the Forest Research Institute Malaysia.
Upon receipt at the laboratory, the seeds were removed manually from the pods.
Fresh seeds were immediately subjected to liquid nitrogen storage and another set of control seeds were kept 20°C cool room for up to six weeks.
Seed samples were withdrawn after 1, 2, 4, and 6 weeks.
Prior to storage and after each storage period, moisture content (MC) and germination of the seeds were checked.
Fresh seeds had 8.3% MC and 93.3% germinated.
Seeds kept under liquid nitrogen temperature (-196°C) had c. 6% MC. However, germination was reduced from 90 to 50% throughout the storage time.
Meanwhile, control seeds showed an increased in MC during the storage, from 7.1 to 8.3%. Germination of the control seeds fell from 90% after one week in storage to 65% after six weeks.
These findings showed that D. costulata seeds tolerated dry storage at both cool (20°C) and ultra-cold (-196°C) temperature for up to six weeks, although the germination percentage decreased under both conditions.
Surviving germinated seeds were transferred to vermiculite for growth evaluation.
Further studies are needed to determine the most suitable seed MC for D. costulata cryopreservation.
Authors
N.A. Nashatul Zaimah, M. Norwati, N. Lokmal
Keywords
Jelutong, seed storage, moisture content, germination, cryopreservation
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