Articles
Arabidopsis – a model to elucidate complex stress response mechanism during cryopreservation
Article number
1234_11
Pages
85 – 96
Language
English
Abstract
Plant cryopreservation is important for genetic resources that cannot maintained as seeds.
For the last decades, sophisticated protocols have been empirically elaborated and comprise shoot tip preparation, osmotic adjustment, cryoprotection, cooling and rewarming.
These treatments are accompanied by multiple stresses, i.e., dehydration, cold and chemical toxicity, that induce reactive oxygen species (ROS). ROS can function as signaling molecules influencing gene, redox and antioxidant regulation and may determine the success of post cryogenic survival.
To decipher the detailed molecular processes during cryopreservation, the potential of the model plant Arabidopsis is used and efficient protocols have been developed.
In particular, plant material can be obtained from homogeneous seeds.
Effects of pre-culture environment and endophytic contamination can be avoided and Arabidopsis shoot tip cryopreservation results in high regrowth percentage after cryo-treatment.
Previous cryopreservation experiments have elucidated that members of the transcription factor families WRKY, MYB and AP2 EREBP, such as DREBs/CBFs are involved in the stress response mechanisms.
Some are known to transduce ROS signals and are participate in complex networks.
Therefore, we may speculate that higher resistance to cryo-stress is related to cross-tolerances with other stresses, such as cold and osmotic stress.
Understanding these specific signaling cascades involved in cryopreservation of Arabidopsis could be useful to improve and develop successful cryopreservation protocols for other plant genetic resources.
For the last decades, sophisticated protocols have been empirically elaborated and comprise shoot tip preparation, osmotic adjustment, cryoprotection, cooling and rewarming.
These treatments are accompanied by multiple stresses, i.e., dehydration, cold and chemical toxicity, that induce reactive oxygen species (ROS). ROS can function as signaling molecules influencing gene, redox and antioxidant regulation and may determine the success of post cryogenic survival.
To decipher the detailed molecular processes during cryopreservation, the potential of the model plant Arabidopsis is used and efficient protocols have been developed.
In particular, plant material can be obtained from homogeneous seeds.
Effects of pre-culture environment and endophytic contamination can be avoided and Arabidopsis shoot tip cryopreservation results in high regrowth percentage after cryo-treatment.
Previous cryopreservation experiments have elucidated that members of the transcription factor families WRKY, MYB and AP2 EREBP, such as DREBs/CBFs are involved in the stress response mechanisms.
Some are known to transduce ROS signals and are participate in complex networks.
Therefore, we may speculate that higher resistance to cryo-stress is related to cross-tolerances with other stresses, such as cold and osmotic stress.
Understanding these specific signaling cascades involved in cryopreservation of Arabidopsis could be useful to improve and develop successful cryopreservation protocols for other plant genetic resources.
Authors
J. Stock, H.P. Mock, A. Senula, M. Nagel
Keywords
cryogenic, gene bank, knock-out mutant, plant genetic resources, plant vitrification solutions, reactive oxygen species, stress response
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