Articles
Cryopreservation of in vitro shoot tips of chayote (Sechium spp.) by D cryo-plate method
Article number
1234_38
Pages
293 – 300
Language
English
Abstract
Maintenance of field collections of chayote (Sechium spp.) is cumbersome and costly.
An alternative method for safe and cost-effective long-term preservation of chayote is required.
An adapted version of the D cryo-plate procedure for long-term preservation of chayote was developed.
Variants of the D cryo-plate procedure were optimized for post-liquid nitrogen (LN) regrowth in chayote shoot tips.
The procedures tested included variation in preculture duration, loading solutions (LSs), duration of LS treatment, air dehydration time, and high temperature exposure.
The optimized procedures were subsequently applied to 11 chayote accessions.
The most effective procedure for high post-LN regrowth consisted of: exposure of shoots to 29°C for 5 days, 2 days preculture on 0.3 M sucrose medium, osmoprotection with LS containing 0.4 M sucrose and 2.0 M glycerol for 45 min, and dehydration for 45 min in Petri dish containing 35 g silica gel.
The optimized procedure resulted in high rates of regrowth for the 11 tested chayote accessions, with regrowth rates ranging from 43.3 to 76.7% and an average of 56.7%. D cryo-plate is a practical and simple procedure for cryo-storage of in vitro grown chayote shoot tips.
An alternative method for safe and cost-effective long-term preservation of chayote is required.
An adapted version of the D cryo-plate procedure for long-term preservation of chayote was developed.
Variants of the D cryo-plate procedure were optimized for post-liquid nitrogen (LN) regrowth in chayote shoot tips.
The procedures tested included variation in preculture duration, loading solutions (LSs), duration of LS treatment, air dehydration time, and high temperature exposure.
The optimized procedures were subsequently applied to 11 chayote accessions.
The most effective procedure for high post-LN regrowth consisted of: exposure of shoots to 29°C for 5 days, 2 days preculture on 0.3 M sucrose medium, osmoprotection with LS containing 0.4 M sucrose and 2.0 M glycerol for 45 min, and dehydration for 45 min in Petri dish containing 35 g silica gel.
The optimized procedure resulted in high rates of regrowth for the 11 tested chayote accessions, with regrowth rates ranging from 43.3 to 76.7% and an average of 56.7%. D cryo-plate is a practical and simple procedure for cryo-storage of in vitro grown chayote shoot tips.
Authors
M. Valle Arizaga, G. Sandoval Cancino, J. Cadena Iñiguez, E.J. Cruz Gutiérrez, L.A. Gómez Reyes, S. Yamamoto, D. Tanaka, S.G. Olvera Vazquez, K. Watanabe, T. Niino
Keywords
air dehydration, chayote, cryopreservation, D cryo-plate, high temperature exposure, Sechium spp
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