Home > XII International Conference on Grapevine Breeding and Genetics > Application and optimization of the CRISPR/Cas9 system in grape

Articles

Application and optimization of the CRISPR/Cas9 system in grape

Article number

1248_22

Pages

149 – 154

Language

English

Abstract

As an innovative and powerful tool for genome engineering, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been quickly applied to a variety of plant species, including several fruit crops.
Through targeted and accurate gene modifications, it enables the development of novel plant cultivars with preferable traits, providing an alternative to plant breeding.
However, many factors such as guide RNA (gRNA) and Cas9 protein impact on its efficiency and specificity, which is a major concern of the CRISPR/Cas9 system during genome editing.
Here, we summarize accomplishments and problems of genome editing mediated by CRISPR/Cas9 in fruit crops, and discuss recent achievements in optimizing the efficiency and specificity of the system.
Accordingly, we provide a basic strategy for genome editing in grape, expecting to hasten the exploration and application of this technology in this species as well as other fruit crops.

Publication

XII International Conference on Grapevine Breeding and Genetics

Authors

C. Ren, E.K. Gathunga, S. Li, Z. Liang

Keywords

application, optimization, CRISPR/Cas9, grape, genome editing, efficiency, basic strategy

Full text

https://doi.org/10.17660/ActaHortic.2019.1248.22

Groups involved

Online Articles (82)

1. Breeding, consumers and market issues: main evolutions in the vine and wine industry

H. Hannin

2. Impact of grapevine breeding for disease resistance on the global wine industry

L. Bavaresco

3. Synonyms and homonyms in Herzegovinian and Dalmatian grapevine cultivars

A. Mandić | M. Žulj Mihaljević | M. Leko | J. Primorac | J. Beljo

4. OSCAR, a national observatory to support the durable deployment of disease-resistant grapevine cultivars

5. Isolation and amplification of ancient DNA from herborized grapevine leaves collected by Spanish botanist Simón de Rojas Clemente y Rubio in 1803-1804

6. Recovering ancient grapevine cultivars in the Spanish provinces of Alicante and Valencia

7. Genetic gains of selection in ancient grapevine cultivars

E. Gonçalves | A. Martins

8. Preservation via utilization: minor grape cultivars on-farm

9. Inventory and descriptions of wild grapevine (Vitis vinifera subsp. sylvestris) from Slovenia, Croatia and Bosnia and Herzegovina

10. Genotyping-by-sequencing-derived single-nucleotide polymorphism catalog from a grapevine (Vitis vinifera L.) germplasm collection that includes the most representative Apulian autochthonous cultivars

11. Genetic variability in grapevine clones of ‘Muscat of Alexandria’

12. Genetic resources for table-grape breeding in Brazilian tropical semi-arid regions

P.C.S. Leão

13. True-to-type confirmation of 20 unknown grapevine accessions at a grape germplasm collection in Split, Croatia

14. New cold-tolerant grapevine cultivars for red wines

Е. Ilnitskaya | T. Guguchkina | A. Talash

15. Postharvest evaluation of cold-hardy table-grape breeding lines

L. Moreira | A. Underhill | M. Clark

16. The Fondazione Edmund Mach grapevine breeding program for downy and powdery mildew resistances: toward a green viticulture

17. Field performance of five white Pilzwiderstandsfahige (PIWI) cultivars in the south of Brazil

18. Rootstock breeding for resistance to grapevine decline and dieback in southern Brazil

M.A. Dalbó | A.L.K. Souza

19. Identification of downy mildew resistance genes Rpv10 and Rpv3 by DNA-marker analysis in a Russian grapevine germplasm collection

Е. Ilnitskaya | S. Tokmakov | M. Makarkina | I. Suprun

20. Phylogeny of Vitis species based on a VvMybA1 marker analysis

A. Kerekes | K.A. Tóth-Lencsés | E. Kiss | A. Szőke

21. Phenology and thermal requirements of disease-resistant genotypes (PIWI) grown in the Goethe Grape Valley region of Brazil

22. Application and optimization of the CRISPR/Cas9 system in grape

C. Ren | E.K. Gathunga | S. Li | Z. Liang

23. Exploring the use of transposon mobilization to produce a gene-tagged population for grapevine

24. Synthesis of grapevine chimeras

25. Toward identification of haplotypes that control seedlessness of grape by genome resequencing

Y.Y. Hur | M.S. Kim | S.C. Jeong

26. In-vitro embryo rescue in table-grape breeding for semiarid tropical regions of Brazil

P.C.S. Leão | N.F. de Melo | B.T.G. Nunes | E.R. da Silva

27. Use of the microvine and plant gene switch system for functional studies of genes involved in the control of ripening initiation in Vitis vinifera

S. Gouthu | L.G. Deluc

28. Generation of mildew-resistant grapevine clones via genome editing

L. Giacomelli | T. Zeilmaker | M. Malnoy | J. Rouppe van der Voort | C. Moser

29. Control of the grapevine moth Lobesia botrana through genetic engineering manipulation of host-plant volatiles

30. INRA-ResDur: the French grapevine breeding programme for durable resistance to downy and powdery mildew

31. Integrating spatial variations in the vineyard to enhance quantitative trait locus (QTL) detection

32. Overview of genetic loci for traits in grapevine and their integration into the VIVC database

L. Hausmann | E. Maul | A. Ganesch | R. Töpfer

33. Genome-wide association study of a diverse grapevine panel: example of berry weight

34. Annotation of whole-plastid genomes of wild grapes (Vitis vinifera subsp. sylvestris) from the south Caucasus, Europe and the Mediterranean Basin

I. Pipia | L. Roma | V. Tabidze

35. Unraveling the key molecular events of grape berry ripening

36. Singular effect of soil and climate on grapevine development and berry traits in two Italian cultivars, ‘Glera’ and ‘Corvina’

C. Perin | M. Lucchin | A. Vannozzi

37. Phenoliner: a multi-sensor field phenotyping platform

38. Phenotyping tools for genetic improvement of table grapes in Chile

P. Cid | M. García | A. Pinolef | P. Barba

39. ‘BRS Vitória’: a new seedless table-grape cultivar for the São Francisco Valley, northeast Brazil

P.C.S. Leão | B.T.G. Nunes | J.H.B. do Nascimento | M.C. de Souza | J.I.S. Rego

40. Molecular characterization of wine grape cultivars from Calabria

41. Whole-genome sequencing and gene annotation of Georgian grape cultivars

V. Tabidze | I. Pipia | M. Gogniashvili | N. Kunelauri | T. Beridze

42. A few main parents contributed to the traditional grapevine cultivar assortment in north-western Italy, as revealed by microsatellites and single-nucleotide polymorphisms

S. Raimondi | P. Ruffa | P. Boccacci | S. Abbà | A. Schneider

43. Extensive genotyping of a large collection of rootstocks, population structure analysis and core collection extrapolation for new breeding programs

44. Quantitative trait locus mapping of downy mildew and botrytis bunch rot resistance in a Vitis aestivalis-derived ‘Norton’-based population

45. Breeding for mildew resistance in grapevine to improve environmental and socio-economic sustainability in hotspot areas of Veneto

B. De Nardi | F. Santellani | T. Possamai | R. Velasco

46. Genetic variation for grapevine reproductive development

47. Molecular analysis of bunch architecture in grapevine

E. Zyprian | R. Richter | S. Rossmann | K. Theres | R. Töpfer

48. Determination of genetic loci in the control network of grapevine flowering

A. Schwandner | I. Ochßner | L. Hausmann | R. Töpfer

49. Mapping the genetic architecture of grapevine bud growth-cycling quantitative traits

A. Fennell | D. Alahakoon | J. Luby | M. Clark

50. Characterization of the reproductive performance of a collection of grapevine cultivars

S. Ibáñez | J. Grimplet | E. Baroja | S. Hernaiz | J. Ibáñez

51. Transcriptomics analysis of ‘Cabernet Sauvignon’ berry skins from Reno and Bordeaux in the late stages of ripening

G.R. Cramer | N. Cochetel | R. Ghan | A. Destrac-Irvine | S. Delrot

52. Developing a model system to identify the main mechanisms involved in nitrogen growth responses of grafted grapevines

L. Rossdeutsch | P. Schreiner | P. Skinkis | L.G. Deluc

53. Understanding scion-rootstock interactions at the graft interface of grapevine

54. Rootstocks for table grape ‘BRS Maria Bonita’ in the São Francisco Valley, northeast Brazil

P.C.S. Leão | M.M.C. de Lira | D.S. de Moraes | E.R. da Silva

55. Rootstocks for table grape ‘BRS Clara’ in the São Francisco Valley, northeast Brazil

P.C.S. Leão | M.M.C. de Lira | D.S. de Moraes | E.R. da Silva

56. Genetic dissection of grape berry ripening control: defining a role for NAC transcription factors

S. Zenoni | E. D¿Incà | G.B. Tornielli

57. Biochemical and transcriptomic analysis in berries of wild and cultivated grapevine (Vitis vinifera L.)

58. Genetic dissection of the natural dry-on-vine (NDOV) trait in grapevine

J. Fresnedo-Ramírez | S.A. Lee | C.A. Ledbetter

59. Prolonged ripening on the vine affects the polyphenolic profile of grapes and wine of ‘Plavac Mali’ (Vitis vinifera L.)

A. Mucalo | G. Zdunić | E. Maletić

60. Methyl jasmonate application to increase volatile compounds of Vitis labrusca L. grape berries cultivated under subtropical conditions

L. Moro | N.T. da Silva | N.M.A. Hassimotto | E. Purgatto

61. Condensed tannins in the pericarp of diverse Vitis species

62. Investigating variability of the cultivar ‘Pinot blanc’ under different aspects

63. Valorization of autochthonous Apulian grapevine cultivars for spumante production

64. Characterization of deletions causing berry-color variation in Garnacha and Tempranillo

65. Grape color variation involves genetic and micro-environmental changes that alter berry phenolic and aromatic composition

66. Proteome analysis to identify anthocyanin transporters in grape cells

67. Can transcriptomics shed light on the “old-vine” character of wines?

B. Coetzee | H.J. Maree | J.T. Burger

68. Grapevine adaptation to abiotic stress: an overview

69. Short sequence repeat (SSR) genotyping and sodium exclusion phenotyping of a Vitis hybrid population (‘K51-40’ × ‘Schwarzmann’)

70. Grapevine rootstocks differentially regulate root growth and architecture in response to nitrogen availability

N. Cochetel | C. Hévin | P. Vivin | N. Ollat | V. Lauvergeat

71. Physiological and biochemical responses to drought stress in some autochthonous grapevines of Turkey

M. Koç | Ö. Kamiloğlu | R. Cangi | K. Yıldız

72. A perspective on breeding and implementing durable powdery mildew resistance

L.E. Cadle-Davidson

73. Pyramidizing resistance genes in grape: a breeding program for the selection of elite cultivars

S. Foria | C. Monte | R. Testolin | G. Di Gaspero | G. Cipriani

74. Use of the NeoViGen96 chip to understand the defense status of cultivars and resistant genotypes of Vitis vinifera

M.F. Corio-Costet | A. Bellée | M.C. Dufour | D. Merdinoglu | C. Coppin

75. Growth and development of Pierce’s disease-tolerant hybrid bunch grapes in the southeastern USA

E.D. Coneva | Y. Hu | A. Svyantek | E. Vinson

76. Subtilisin-like proteins and lipid signalling events: the missing links in grapevine resistance to Plasmopara viticola

77. Early detection of Plasmopara viticola-infected leaves through FT-ICR-MS metabolic profiling

78. The Rpv3-3 haplotype and stilbenoid induction mediate downy mildew resistance in a grapevine interspecific population

79. The molecular dialogue between grapevine inflorescence/berry and Botrytis cinerea during initial, quiescent and egression infection stages

80. Durable powdery mildew resistance in grapevines: myth or reality

S. Riaz | A. Tenscher | D. Pap | N. Romero | M.A. Walker

81. Contrasting susceptibilities to Flavescence dorée in Vitis vinifera cultivars and progenies suggest segregation of genetic traits involved in disease response

82. Evidence for sexual reproduction and fertile oospore production by Plasmopara viticola on the leaves of partially resistant grapevine cultivars

L. Delbac | L. Delière | C. Schneider | F. Delmotte