Home > XII International Conference on Grapevine Breeding and Genetics > Whole-genome sequencing and gene annotation of Georgian grape cultivars

Articles

Whole-genome sequencing and gene annotation of Georgian grape cultivars

Article number

1248_41

Pages

287 – 294

Language

English

Abstract

The genomes of four Georgian grape cultivars, Chkhaveri, Saperavi, Meskhetian green, and Rkatsiteli, belonging to different haplogroups, were resequenced.
Shotgun genomic libraries of grape cultivars were sequenced on an Illumina HiSeq.
The sequences of all 19 chromosomes of the four Georgian grape cultivars were determined by resequencing using Pinot noir as a reference genome.
Annotation of chromosomal DNA of Georgian grape revealed 17,409 candidate genes in Chkhaveri, 17,021 in Saperavi, 18,355 in Meskhetian green and 13,960 in Rkatsiteli. Among them, 106 predicted genes and 43 pseudogenes of terpene synthase (TPS) and 44 candidate genes of stilbene synthase (STS) were found in all studied Georgian cultivars.
Several additional TPS and STS genes not present in the reference Pinot noir genome were detected.
This is the first attempt at a comparative whole-genome analysis of different haplogroups of Vitis vinifera cultivars.

Publication

XII International Conference on Grapevine Breeding and Genetics

Authors

V. Tabidze, I. Pipia, M. Gogniashvili, N. Kunelauri, T. Beridze

Keywords

grape genome, gene annotation, Illumina sequencing, terpene synthase, stilbene synthase

Full text

https://doi.org/10.17660/ActaHortic.2019.1248.41

Groups involved

Online Articles (82)

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