Articles
Improvement of the rooting of micropropagated Pistacia terebinthus L.
Article number
1259_23
Pages
143 – 148
Language
English
Abstract
Pistacia terebinthus L. is widely spread in the Mediterranean region and has significant importance as a wild species and a rootstock because of its exceptional drought resistance which allows cultivation on low productive and stony soils.
Prof.
Avanzato discovered an unusual monoecious form in the Rhodopes Mountains about 15 years ago.
We have developed an effective protocol for micropropagation of this unique form.
As with most woody species, in Pistacia rooting is a critical step in micropropagation and the quality of the root system is the main factor that affects survival and growth of plantlets under ex vitro conditions.
The aim of the present study was to improve the rooting of micropropagated monoecious plants by applying a new plant growth biostimulator of natural origin, Charkor.
Charkor contains metabolism products of symbiotic fungus-endophyte of ginseng roots, cultivated in vitro.
The plant material was maintained on a solidified DKW nutrient medium supplemented with 2.5 μM BAP, 0.005 μM IBA and 30 g L‑1 glucose.
The shootlets obtained during multiplication were elongated on hormone-free DKW nutrient medium for 10 days.
In the rooting stage Charkor was tested alone or in combination with IBA and NAA. The best result (75% of rooted plantlets) was obtained with plants treated with combination of 10 µM IBA, 0.054 µM NAA and 1 mL L‑1 Charkor.
Prof.
Avanzato discovered an unusual monoecious form in the Rhodopes Mountains about 15 years ago.
We have developed an effective protocol for micropropagation of this unique form.
As with most woody species, in Pistacia rooting is a critical step in micropropagation and the quality of the root system is the main factor that affects survival and growth of plantlets under ex vitro conditions.
The aim of the present study was to improve the rooting of micropropagated monoecious plants by applying a new plant growth biostimulator of natural origin, Charkor.
Charkor contains metabolism products of symbiotic fungus-endophyte of ginseng roots, cultivated in vitro.
The plant material was maintained on a solidified DKW nutrient medium supplemented with 2.5 μM BAP, 0.005 μM IBA and 30 g L‑1 glucose.
The shootlets obtained during multiplication were elongated on hormone-free DKW nutrient medium for 10 days.
In the rooting stage Charkor was tested alone or in combination with IBA and NAA. The best result (75% of rooted plantlets) was obtained with plants treated with combination of 10 µM IBA, 0.054 µM NAA and 1 mL L‑1 Charkor.
Authors
L.R. Nacheva, P.S. Gercheva, A.T. Zhivondov, S.M. Malchev
Keywords
micropropagation, shoot culture, in vitro, biostimulator, Charkor
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