Articles
Changes in polyamine levels on sunburn apple skin
Article number
1261_39
Pages
273 – 280
Language
English
Abstract
Polyamines (PAs) participate in the defense reaction against several environmental conditions that causes oxidative stress.
Solar radiation stress is common in many fruit-growing regions of the world and produces an apple physiological disorder called sunburn which causes severe crop loss every year.
The aim of this work was to study PAs role in sunburn apples skin in North Patagonia. ‘Red Delicious’ apples maturity indices were determined at harvest time in healthy (HS) and slight sunburn side (SS) of the fruit.
Skin tissues from both sides were evaluated in terms of, lipid peroxidation (thiobarbituric acid-reactive substances, TBARs), antioxidant activity (DPPH radical-scavenging), and free PAs content.
Flesh firmness was higher in SS than HS and no differences were found in soluble solid, starch index and tritatable acidity.
Lipid peroxidation was higher in SS (80.8 nmol g‑1 FW) than in HS (53.1 nmol g‑1 FW). DPPH was 113% higher (p<0.05) in SS than HS. A significant increase of total PAs level was observed in SS in regard to HS. Free putrescina was the most abundant PAs in both tissues.
Putrescine concentration was 206.8 nmol g‑1 FW in HS and 352.4 nmol g‑1 FW in SS. Spermidine level were 93.0 and 129.6 nmol g‑1 FW for HS and SS, respectively.
No differences were found in spermine content between tissues.
Sunburn injury triggered membranes damage raise and a response of the apple skin antioxidant system.
Accumulation of putrescine and spermidine were observed under solar radiation stress.
However, this increase in PAs content and antioxidant metabolites were not effective to alleviate oxidative damage and apple sunburn symptoms.
Further information is needed to understand the PAs role in apples skin under solar radiation stress.
Solar radiation stress is common in many fruit-growing regions of the world and produces an apple physiological disorder called sunburn which causes severe crop loss every year.
The aim of this work was to study PAs role in sunburn apples skin in North Patagonia. ‘Red Delicious’ apples maturity indices were determined at harvest time in healthy (HS) and slight sunburn side (SS) of the fruit.
Skin tissues from both sides were evaluated in terms of, lipid peroxidation (thiobarbituric acid-reactive substances, TBARs), antioxidant activity (DPPH radical-scavenging), and free PAs content.
Flesh firmness was higher in SS than HS and no differences were found in soluble solid, starch index and tritatable acidity.
Lipid peroxidation was higher in SS (80.8 nmol g‑1 FW) than in HS (53.1 nmol g‑1 FW). DPPH was 113% higher (p<0.05) in SS than HS. A significant increase of total PAs level was observed in SS in regard to HS. Free putrescina was the most abundant PAs in both tissues.
Putrescine concentration was 206.8 nmol g‑1 FW in HS and 352.4 nmol g‑1 FW in SS. Spermidine level were 93.0 and 129.6 nmol g‑1 FW for HS and SS, respectively.
No differences were found in spermine content between tissues.
Sunburn injury triggered membranes damage raise and a response of the apple skin antioxidant system.
Accumulation of putrescine and spermidine were observed under solar radiation stress.
However, this increase in PAs content and antioxidant metabolites were not effective to alleviate oxidative damage and apple sunburn symptoms.
Further information is needed to understand the PAs role in apples skin under solar radiation stress.
Publication
Authors
L.I. Vita, S.J. Maiale, N. Spera, G.M. Colavita
Keywords
high solar radiation, oxidative stress, abiotic stress, antioxidants
Groups involved
Online Articles (39)