Articles
Self (in)compatibility and Plum pox virus resistance in Pakistan apricots (Prunus armeniaca)
Article number
1290_13
Pages
69 – 76
Language
English
Abstract
Apricot (Prunus armeniaca L.) is an important food source in Gilgit-Baltistan region of Pakistan, where a rich apricot cultivation has been continued for centuries.
Here we have characterized 63 accessions from Gilgit-Baltistan examining self (in)compatibility (SI) loci and Plum pox virus (PPV) resistance.
Additionally, recently reported SSRs for identification of M loci related with self-incompatibility in apricots were examined.
Among the 21 S-RNase alleles have been described in European apricots, S12 (25%), S9 (19%), S2 (16%), S6 (9%), S3 (6%), S13 (6%), S20 (4%) and S7 (2%) were identified for Gilgit-Baltistan accessions.
A fragment with a size of 355 nucleotides (nt) for the first intron of S-RNase gene previously attributed to the self compatibility (SC) and S8-RNase alleles in European apricots was observed only for one Pakistani accession.
On contrary, an insertion of 358 nt in the SFB gene which is indicator of SC haplotype was harbored by 36 accessions, indicating that Pakistani accessions may not fit to the molecular explanations hypothesized for SC loci in European apricot accessions.
Regarding to genotyping M loci, alleles of 7 SSR loci were amplified successfully but none of SSR loci profile of the accessions was attributed to previously discovered M-loci alleles.
Four markers (PGS1.21 PGS1.23, PGS1.24, and ZP002) showing allelic variants associated with PPV resistance were investigated in Pakistani accessions.
Interestingly, in contrast to previous findings, no resistance linked marker was identified in Pakistani apricots.
Here we have characterized 63 accessions from Gilgit-Baltistan examining self (in)compatibility (SI) loci and Plum pox virus (PPV) resistance.
Additionally, recently reported SSRs for identification of M loci related with self-incompatibility in apricots were examined.
Among the 21 S-RNase alleles have been described in European apricots, S12 (25%), S9 (19%), S2 (16%), S6 (9%), S3 (6%), S13 (6%), S20 (4%) and S7 (2%) were identified for Gilgit-Baltistan accessions.
A fragment with a size of 355 nucleotides (nt) for the first intron of S-RNase gene previously attributed to the self compatibility (SC) and S8-RNase alleles in European apricots was observed only for one Pakistani accession.
On contrary, an insertion of 358 nt in the SFB gene which is indicator of SC haplotype was harbored by 36 accessions, indicating that Pakistani accessions may not fit to the molecular explanations hypothesized for SC loci in European apricot accessions.
Regarding to genotyping M loci, alleles of 7 SSR loci were amplified successfully but none of SSR loci profile of the accessions was attributed to previously discovered M-loci alleles.
Four markers (PGS1.21 PGS1.23, PGS1.24, and ZP002) showing allelic variants associated with PPV resistance were investigated in Pakistani accessions.
Interestingly, in contrast to previous findings, no resistance linked marker was identified in Pakistani apricots.
Authors
M.A. Köse, N. Çetinsağ, H. Khan, K. Gürcan, S. Ercişli
Keywords
genetic diversity, simple sequence repeats, microsatellite
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