Articles
Proteas micropropagation a review
Article number
1347_1
Pages
1 – 14
Language
English
Abstract
Micropropagation of Proteaceae species could be an alternative to satisfy commercial demand as well as to provide plant health guarantees for international trade.
The main research about micropropagation of Proteaceae is analyzed in this review paper, based on the literature obtained from the past four decades.
The following main problems for in vitro culture of proteas are discussed: 1) Phenolic oxidation of primary explants is an important factor during the initiation stage of several Proteaceae species making growth and development too difficult or impossible. 2) A low growth of explants in the establishment stage (stage I) and low proliferation rates during multiplication stage (stage II) of some species and cultivars can require to include elongation stages. 3) In vitro rooting (stage III) can be difficult and several species and cultivars have been shown reduced rooting rates. 4) Acclimatization problems of microcuttings (stage IV) can be extremely important and 5) Poor performance in tissue culture of some rare and threatened species in which young plant material from mature plants are extremely difficult or currently impossible to initiate into culture.
Focused attention is given to axillary or terminal buds proliferation methods and several approaches from our laboratory using forced solutions as a pre-treatment of mother plants (stage 0). Preliminary results concerning to the addition of macronutrients that the leaves contain in the period of maximum growth to the culture medium are presented and discussed and structural and ultraestructural changes of plantlets during transfer from in vitro to ex vitro environment are also described.
The main research about micropropagation of Proteaceae is analyzed in this review paper, based on the literature obtained from the past four decades.
The following main problems for in vitro culture of proteas are discussed: 1) Phenolic oxidation of primary explants is an important factor during the initiation stage of several Proteaceae species making growth and development too difficult or impossible. 2) A low growth of explants in the establishment stage (stage I) and low proliferation rates during multiplication stage (stage II) of some species and cultivars can require to include elongation stages. 3) In vitro rooting (stage III) can be difficult and several species and cultivars have been shown reduced rooting rates. 4) Acclimatization problems of microcuttings (stage IV) can be extremely important and 5) Poor performance in tissue culture of some rare and threatened species in which young plant material from mature plants are extremely difficult or currently impossible to initiate into culture.
Focused attention is given to axillary or terminal buds proliferation methods and several approaches from our laboratory using forced solutions as a pre-treatment of mother plants (stage 0). Preliminary results concerning to the addition of macronutrients that the leaves contain in the period of maximum growth to the culture medium are presented and discussed and structural and ultraestructural changes of plantlets during transfer from in vitro to ex vitro environment are also described.
Publication
Authors
J.F. Pérez-Francés
Keywords
Proteaceae, in vitro, shoot multiplication, rooting, acclimatization
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