Articles
In vitro thermotherapy combined with chemotherapy-based methods for Cnidium officinale Makino virus elimination
Article number
1358_11
Pages
79 – 84
Language
English
Abstract
Cnidium officinale is used for traditional medicinal plants and accounts for more than 10% of the herbal medicine market in Korea.
The C. officinale do not produce seeds, so proliferation takes place as tuberous roots.
However, in commercial crops of C. officinale, Cucumber mosaic virus (CMV) and Apple stem growing virus (ASGV) are common pathogens that can significantly reduce the yield and quality of tuberous roots We used a cultivar of C. officinale widely grown in Korea, infected with CMV and ASGV. We carried out the effectiveness of chemotherapy with different concentrations of ribavirin, thermotherapy at different temperatures, and combinations of these two methods on virus elimination.
In this procedure, infected explants were regenerated on MS medium containing ribavirin at concentrations of 10, 20, and 40 mg L-1 for 4-week, followed by a thermotherapy treatment for 15 days at 25~37°C. The survival and regeneration of plants were evaluated, and the efficiency of virus eradication was determined by reverse- transcription polymerase chain reaction with two primer pairs for each virus.
Ribavirin treatments at 40 mg L-1 of CMV and ASGV resulted in virus elimination rates of 89.5 and 94.7%, respectively, although the survival rate was rapidly decreased The average regeneration rate of virus-free plants after high temperature treatments (33~37°C) for 15 days decreased to less than 20%. The virus elimination efficiency of CMV and ASGV was enhanced by combining 20 mg L-1 ribavirin and thermotherapy at 33°C resulting in high virus eradication efficiency (100 and 96%) better than single treatments.
The results showed that the combined treatments were highly effective in eliminating CMV and ASGV.
The C. officinale do not produce seeds, so proliferation takes place as tuberous roots.
However, in commercial crops of C. officinale, Cucumber mosaic virus (CMV) and Apple stem growing virus (ASGV) are common pathogens that can significantly reduce the yield and quality of tuberous roots We used a cultivar of C. officinale widely grown in Korea, infected with CMV and ASGV. We carried out the effectiveness of chemotherapy with different concentrations of ribavirin, thermotherapy at different temperatures, and combinations of these two methods on virus elimination.
In this procedure, infected explants were regenerated on MS medium containing ribavirin at concentrations of 10, 20, and 40 mg L-1 for 4-week, followed by a thermotherapy treatment for 15 days at 25~37°C. The survival and regeneration of plants were evaluated, and the efficiency of virus eradication was determined by reverse- transcription polymerase chain reaction with two primer pairs for each virus.
Ribavirin treatments at 40 mg L-1 of CMV and ASGV resulted in virus elimination rates of 89.5 and 94.7%, respectively, although the survival rate was rapidly decreased The average regeneration rate of virus-free plants after high temperature treatments (33~37°C) for 15 days decreased to less than 20%. The virus elimination efficiency of CMV and ASGV was enhanced by combining 20 mg L-1 ribavirin and thermotherapy at 33°C resulting in high virus eradication efficiency (100 and 96%) better than single treatments.
The results showed that the combined treatments were highly effective in eliminating CMV and ASGV.
Authors
J.A. Kim, H.Y. Jeong
Keywords
ASGV, CMV, Cnidium officinale, virus elimination, virus-free plants
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