Articles
Total phenols, flavonoids, proanthocyanidins and antioxidant potential of Justicia secunda Vahl
Article number
1358_28
Pages
211 – 218
Language
English
Abstract
Justicia secunda Vahl belongs to the family Acanthaceae. The plant was primarily cultivated for ornamental purposes before the discovery of its medicinal uses for the treatment of chronic diseases threatening public health.
In the present study, extraction of the leaves of the plant was carried out using organic solvents (acetone, chloroform and ethanol), and the total phenols, flavonoids, and proanthocyanidin in the crude extracts were estimated.
The antioxidant potentials of the extracts were also investigated in vitro using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) method.
The highest yields of phenols (30.35±1.21 GAE g-1), flavonoids (155.21±40.52 QE g-1) and proanthocyanidin (37.65±5.26 CE g-1) were recorded in acetone extracts, followed by ethanol extracts having (26.56±0.70 GAE g-1) (total phenols), 135.18±24.82 QE g-1 (flavonoids) and no proanthocyanidin while the lowest yields of phenols (80.27±0.94 GAE g-1) and flavonoids (95.27±2.85 QE g-1) with no proanthocyanidin were recorded in the chloroform extract.
With acetone extract, the DPPH antioxidant assay was not concentration-dependent across the tested concentration ranges (0.025-0.2 mg mL-1), as observed with other solvents.
This indicates that the DPPH assay may not be appropriate for the determination of the antioxidant activity of J. secunda owing to the high anthocyanin content in the plant that may have interfered with the colorimetric assay.
Findings from this study also showed that the detetcted phytochemical composition of the plants was influenced by solvent polarity.
Hence, other antioxidant assays are recommended to assess the antioxidant potential of the plant.
In the present study, extraction of the leaves of the plant was carried out using organic solvents (acetone, chloroform and ethanol), and the total phenols, flavonoids, and proanthocyanidin in the crude extracts were estimated.
The antioxidant potentials of the extracts were also investigated in vitro using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) method.
The highest yields of phenols (30.35±1.21 GAE g-1), flavonoids (155.21±40.52 QE g-1) and proanthocyanidin (37.65±5.26 CE g-1) were recorded in acetone extracts, followed by ethanol extracts having (26.56±0.70 GAE g-1) (total phenols), 135.18±24.82 QE g-1 (flavonoids) and no proanthocyanidin while the lowest yields of phenols (80.27±0.94 GAE g-1) and flavonoids (95.27±2.85 QE g-1) with no proanthocyanidin were recorded in the chloroform extract.
With acetone extract, the DPPH antioxidant assay was not concentration-dependent across the tested concentration ranges (0.025-0.2 mg mL-1), as observed with other solvents.
This indicates that the DPPH assay may not be appropriate for the determination of the antioxidant activity of J. secunda owing to the high anthocyanin content in the plant that may have interfered with the colorimetric assay.
Findings from this study also showed that the detetcted phytochemical composition of the plants was influenced by solvent polarity.
Hence, other antioxidant assays are recommended to assess the antioxidant potential of the plant.
Authors
M.O. Jimoh, M.A. Jimoh, O.O. Bakare, S.O. Bamigboye, C.T. Senjobi, A. Sogoni, K. Okaiyeto, L. Kambizi, C.P. Laubscher
Keywords
2,2-diphenyl-1-picryl-hydrazyl (DPPH), Acanthaceae, bioactive compounds, Justicia secunda, phytochemicals
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