Articles
Preliminary study of encapsulation in silk fibroin nanoparticles of phenolic compounds from Salvia lavandulifolia Vahl. distillation residues
Article number
1358_31
Pages
235 – 242
Language
English
Abstract
Salvia lavandulifolia Vahl. is an aromatic and medicinal plant native to the western Mediterranean basin.
Its commercial interest mainly focuses on extraction of its essential oil, although it is well known that its distillation residues are a source of phenolic components with many biological properties.
One of the main obstacles in the use of these phenolic components is their lack of stability against external potential oxidants.
To solve this problem, we propose their encapsulation in the silk fibroin nanoparticles (SFN) due to its capacity to act as a bioactive carrier and stabilizer of molecules.
Thus, the main objective of this work was to determine the most suitable ratio among silk fibroin nanoparticles (SFN) and the hydro-soluble sage extract (HSE) in order to optimize the encapsulation efficiency (EE%) and the polyphenolic loading content (PLC). For this, four different ratios were tested: 1:2, 1:4, 1:8 and 1:16 of HSE/SFN (w/w). The loaded nanoparticles were characterized in terms of hydrodynamic size (Zaverage), Polydispersity (PDI), Z-potential (ζ), crystallinity of the protein, EE% and DLC. Main results showed that the size of the loaded nanoparticles increased slightly from 143.8 to 152.8 nm, remaining a monodisperse size distribution (PDI varied among 0.124 and 0.135) and the ζ shifted from -27.7 to -31.7 mV when the loading of polyphenols increased.
The secondary structure of the silk fibroin in the nanoparticles was not affected by the incubation and loading, remaining mainly in beta sheet configuration.
HPLC analysis confirmed the presence of salvianic acid, rosmarinic acid, luteolin7-O-glucoronide and apigenin-7-O-glucoronide as the major components retained by the SFN. Values of EE% (0.7-1.9, 7-24, 11.2-22.5 and 16.5-26.5% respectively) and DLC (10-40 µg g-1, 620-1480 µg g-1, 200-800 µg g-1, 50-270 µg g-1, respectively) pointed to an optimized ratio 1:2 (HSE/SFN).
Its commercial interest mainly focuses on extraction of its essential oil, although it is well known that its distillation residues are a source of phenolic components with many biological properties.
One of the main obstacles in the use of these phenolic components is their lack of stability against external potential oxidants.
To solve this problem, we propose their encapsulation in the silk fibroin nanoparticles (SFN) due to its capacity to act as a bioactive carrier and stabilizer of molecules.
Thus, the main objective of this work was to determine the most suitable ratio among silk fibroin nanoparticles (SFN) and the hydro-soluble sage extract (HSE) in order to optimize the encapsulation efficiency (EE%) and the polyphenolic loading content (PLC). For this, four different ratios were tested: 1:2, 1:4, 1:8 and 1:16 of HSE/SFN (w/w). The loaded nanoparticles were characterized in terms of hydrodynamic size (Zaverage), Polydispersity (PDI), Z-potential (ζ), crystallinity of the protein, EE% and DLC. Main results showed that the size of the loaded nanoparticles increased slightly from 143.8 to 152.8 nm, remaining a monodisperse size distribution (PDI varied among 0.124 and 0.135) and the ζ shifted from -27.7 to -31.7 mV when the loading of polyphenols increased.
The secondary structure of the silk fibroin in the nanoparticles was not affected by the incubation and loading, remaining mainly in beta sheet configuration.
HPLC analysis confirmed the presence of salvianic acid, rosmarinic acid, luteolin7-O-glucoronide and apigenin-7-O-glucoronide as the major components retained by the SFN. Values of EE% (0.7-1.9, 7-24, 11.2-22.5 and 16.5-26.5% respectively) and DLC (10-40 µg g-1, 620-1480 µg g-1, 200-800 µg g-1, 50-270 µg g-1, respectively) pointed to an optimized ratio 1:2 (HSE/SFN).
Authors
A.A. Lozano-Pérez, G. Cáceres-Cevallos, C. Martínez-Conesa, M. Quílez-Simón, I. García-Aledo, P. Romero-Espinar, C. Power, M.J. Jordán
Keywords
Spanish sage extracts, post-distilled byporducts, nanoencapsulation, encapsulation efficiency, polyphenolic loading content
Online Articles (44)