Articles
Analysis of R2R3-MYB transcription factor family in Ficus carica L. identified FcMYB57 as a negative regulator in anthocyanin biosynthesis
Article number
1405_14
Pages
115 – 136
Language
English
Abstract
MYB transcription factors are one of the largest transcription factor families in plants and play a critical regulatory role in anthocyanin biosynthesis.
In this study, a total of 113 R2R3-MYB genes were identified from the genome and transcriptome in fig and selected the main effective inhibitor of R2R3-MYB, FcMYB57, by combining phylogenetic analysis and expression characteristics which showed a significantly negative correlation with the expression of anthocyanin biosynthesis genes.
Yeast one-hybrid and fluorescence enzyme assays confirmed that FcMYB57 significantly inhibited the structural gene UFGT in the anthocyanin biosynthesis pathway in fig.
Yeast two-hybrid experiments did not identify any bHLH (basic helix-loop-helix) and WD40 proteins that interacted with FcMYB57, but revealed an interaction relationship between FcMYB57 and FcTPL (TOPLESS) protein in figs.
Transient expression of FcMYB57 in strawberry fruits showed a significant phenotype of inhibiting anthocyanin accumulation, and qRT-PCR confirmed that the expression levels of structural genes in the anthocyanin biosynthesis pathway of strawberry were all downregulated.
Metabolomic analysis of the treated strawberry tissues confirmed that the content of anthocyanins, including cyanidin-3-O-glucoside and pelargonidin-3-O-glucoside, was significantly reduced, while the content of other flavonoids was also decreased.
Transcriptome data of the strawberry tissues showed that the expression levels of structural genes in the anthocyanin biosynthesis pathway were downregulated.
This study broadened our understanding of the inhibitory effect of R2R3-MYB inhibitors on anthocyanin biosynthesis in figs.
In this study, a total of 113 R2R3-MYB genes were identified from the genome and transcriptome in fig and selected the main effective inhibitor of R2R3-MYB, FcMYB57, by combining phylogenetic analysis and expression characteristics which showed a significantly negative correlation with the expression of anthocyanin biosynthesis genes.
Yeast one-hybrid and fluorescence enzyme assays confirmed that FcMYB57 significantly inhibited the structural gene UFGT in the anthocyanin biosynthesis pathway in fig.
Yeast two-hybrid experiments did not identify any bHLH (basic helix-loop-helix) and WD40 proteins that interacted with FcMYB57, but revealed an interaction relationship between FcMYB57 and FcTPL (TOPLESS) protein in figs.
Transient expression of FcMYB57 in strawberry fruits showed a significant phenotype of inhibiting anthocyanin accumulation, and qRT-PCR confirmed that the expression levels of structural genes in the anthocyanin biosynthesis pathway of strawberry were all downregulated.
Metabolomic analysis of the treated strawberry tissues confirmed that the content of anthocyanins, including cyanidin-3-O-glucoside and pelargonidin-3-O-glucoside, was significantly reduced, while the content of other flavonoids was also decreased.
Transcriptome data of the strawberry tissues showed that the expression levels of structural genes in the anthocyanin biosynthesis pathway were downregulated.
This study broadened our understanding of the inhibitory effect of R2R3-MYB inhibitors on anthocyanin biosynthesis in figs.
Publication
Authors
Z. Wang, H.T. Huang, M.Y. Song, H.Y. He, H.Q. Ma
Keywords
Ficus carica L., R2R3-MYB, repressor, anthocyanin
Groups involved
Online Articles (22)