Articles
Auxin transport in axillary buds of Petunia × atkinsiana treated by mechanical stimulation
Article number
1426_75
Pages
553 – 558
Language
English
Abstract
Mechanical stimulation (MS) refers to the plant’s response to natural stimuli like wind, heavy rain, or touch, leading to a decrease in length, as described by Jaffe in 1973. Several hypotheses have been proposed to explain how MS influences plant growth at the cellular level.
These theories suggest that MS could increase radial expansion while inhibiting plant growth by disrupting auxin synthesis or transport.
Current studies suggest that mechanical stimulation might hinder auxin synthesis in shoot apex and young leaves and/or its basipetal transport.
In our present study, we aimed to investigate whether MS truly affects auxin synthesis or transport during the early stages of plant growth.
Our research also explored the physiological factors responsible for growth arrest in petunia plants subjected to mechanical stimulation.
The plant material, Petunia × atkinsiana, ‘Pegasus® Special Burgundy Bicolor’ with purple-white rays on the petals, was obtained from the Volmary Polska Company, central Poland, Mazovia) as 4-week-old plantlets at the beginning of February.
Free IAA was extracted with 80% methanol and assayed spectrophotometrically with the Salkowski reagent.
Immunolocalization of AUX1, LAX1, and PIN1 proteins, was performed on longitudinal stem segments of the apex (SAM) and axillary buds 1 cm below SAM as well as apical parts of roots (RAM). The anti-Arabidopsis-PIN1, AUX1, and LAX1 antibodies (Sigma Aldrich – Merck, Poland). The mechanical stimutation of plants inhibits the auxin synthesis of the studied plants, but over a longer period of treatment.
The first significant decrease of IAA content was observed on 30 day of experiment.
Mechanical stimulation does not arrest IAA transport.
These theories suggest that MS could increase radial expansion while inhibiting plant growth by disrupting auxin synthesis or transport.
Current studies suggest that mechanical stimulation might hinder auxin synthesis in shoot apex and young leaves and/or its basipetal transport.
In our present study, we aimed to investigate whether MS truly affects auxin synthesis or transport during the early stages of plant growth.
Our research also explored the physiological factors responsible for growth arrest in petunia plants subjected to mechanical stimulation.
The plant material, Petunia × atkinsiana, ‘Pegasus® Special Burgundy Bicolor’ with purple-white rays on the petals, was obtained from the Volmary Polska Company, central Poland, Mazovia) as 4-week-old plantlets at the beginning of February.
Free IAA was extracted with 80% methanol and assayed spectrophotometrically with the Salkowski reagent.
Immunolocalization of AUX1, LAX1, and PIN1 proteins, was performed on longitudinal stem segments of the apex (SAM) and axillary buds 1 cm below SAM as well as apical parts of roots (RAM). The anti-Arabidopsis-PIN1, AUX1, and LAX1 antibodies (Sigma Aldrich – Merck, Poland). The mechanical stimutation of plants inhibits the auxin synthesis of the studied plants, but over a longer period of treatment.
The first significant decrease of IAA content was observed on 30 day of experiment.
Mechanical stimulation does not arrest IAA transport.
Publication
Authors
N. Kuźma, A. Jędrzejuk, M. Serek
Keywords
mechanical stimulation, auxin synthesis, auxin transport
Groups involved
- Division Precision Horticulture and Engineering
- Division Greenhouse and Indoor Production Horticulture
- Working Group Nettings in Horticulture (subgroup of Protected Cultivation in Mild Winter Climates)
- Working Group Light in Horticulture
- Working Group Organic Greenhouse Horticulture
- Working Group Vegetable Grafting
- Working Group Modelling Plant Growth, Environmental Control, Greenhouse Environment
- Working Group Protected Cultivation, Nettings and Screens for Mild Climates
- Working Group Computational Fluid Dynamics in Agriculture
- Working Group Design and Automation in Integrated Indoor Production Systems
- Working Group Mechanization, Digitization, Sensing and Robotics
- Working Group Greenhouse Environment and Climate Control
- Division Landscape and Urban Horticulture
- Commission Agroecology and Organic Farming Systems
- Division Vegetables, Roots and Tubers
- Working Group Vertical Farming
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