Articles
AcMYB88 positively regulates anthocyanin biosynthesis in kiwifruit
Article number
1431_53
Pages
415 – 422
Language
English
Abstract
MYB transcription factors play an important role in anthocyanin accumulation of kiwifruit.
Identifying MYB transcription factors that regulate anthocyanin biosynthesis and verifying gene functions facilitate elucidating the molecular mechanism of anthocyanin accumulation.
It was reported that AcMYB110 was a key transcription factor promoting anthocyanin accumulation in kiwifruit.
In PlantTFDB database, 12 MYB genes that showing high homology to AcMYB110 were identified from 97 kiwifruit MYB genes.
The results revealed that the 12 MYB transcription factors encoded amino acids ranging from 200 to 423; their molecular weight ranged from 22.3 to 45.5 kDa, and all of them were hydrophilic proteins located in the nucleus.
Among the 12 MYB transcription factors, the expression pattern of AcMYB88 gene was very similar to that of AcMYB110 gene during kiwifruit development.
Transient overexpression experiments in tobacco demonstrated that AcMYB88 acts as a positive regulator of anthocyanin accumulation, and AcMYB88 synergistically promoted anthocyanin accumulation with AcMYB110 and AcbHLH42. This study facilitates elucidating the molecular mechanism of anthocyanin accumulation and provides theoretical foundation for breeding research in kiwifruit.
Identifying MYB transcription factors that regulate anthocyanin biosynthesis and verifying gene functions facilitate elucidating the molecular mechanism of anthocyanin accumulation.
It was reported that AcMYB110 was a key transcription factor promoting anthocyanin accumulation in kiwifruit.
In PlantTFDB database, 12 MYB genes that showing high homology to AcMYB110 were identified from 97 kiwifruit MYB genes.
The results revealed that the 12 MYB transcription factors encoded amino acids ranging from 200 to 423; their molecular weight ranged from 22.3 to 45.5 kDa, and all of them were hydrophilic proteins located in the nucleus.
Among the 12 MYB transcription factors, the expression pattern of AcMYB88 gene was very similar to that of AcMYB110 gene during kiwifruit development.
Transient overexpression experiments in tobacco demonstrated that AcMYB88 acts as a positive regulator of anthocyanin accumulation, and AcMYB88 synergistically promoted anthocyanin accumulation with AcMYB110 and AcbHLH42. This study facilitates elucidating the molecular mechanism of anthocyanin accumulation and provides theoretical foundation for breeding research in kiwifruit.
Publication
Authors
Q. Zhang, X.Y. Yuan, Caihong Zhong
Keywords
anthocyanin, kiwifruit, molecular mechanism
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