Articles
Dissecting genomic diversity and nutritional profiles in cultivated rocket salad (Eruca sativa) germplasm
Article number
1442_25
Pages
191 – 198
Language
English
Abstract
Cultivated rocket (Eruca sativa Mill.) is an herbaceous crop with aromatic leaves grown all over the world and consumed as salad.
In this study, 56 cultivated rocket accessions have been investigated for their genomic diversity and for the variable levels of vitamin C and main sugars including glucose, fructose and saccharose.
Double digest restriction associated DNA sequencing has been adopted for the investigation of genetic diversity allowing to detect over 40,000 genome wide SNP markers.
The population structure revealed genomic footprints by detecting subpopulations with different geographical origins that separated European and American accessions from the Asian and Africa ones.
Biochemical analysis showed a great variability in the bioactive component content of leaves.
The average ascorbic acid concentration was 2.16 mg g‑1 fresh weight (f.w.) with values ranging from 0.01- 4.06 mg g‑1 f.w.
Similarly, the average sugar content was 8.04 mg g‑1 f.w., with a range of 1.17 to 15.37 mg g‑1 f.w.
The metabolic diversity of the collection highlights a greater variability among the genotypes selected in the Mediterranean area compared to those with Asian and African origins.
The current rocket salad collection is being expanded to build a larger germplasm core-set for genome-wide association studies toward the dissection of the genetic basis of quality traits.
The findings and tools produced in this research represent a unique opportunity to revolutionize the breeding strategies in rocket salad.
In this study, 56 cultivated rocket accessions have been investigated for their genomic diversity and for the variable levels of vitamin C and main sugars including glucose, fructose and saccharose.
Double digest restriction associated DNA sequencing has been adopted for the investigation of genetic diversity allowing to detect over 40,000 genome wide SNP markers.
The population structure revealed genomic footprints by detecting subpopulations with different geographical origins that separated European and American accessions from the Asian and Africa ones.
Biochemical analysis showed a great variability in the bioactive component content of leaves.
The average ascorbic acid concentration was 2.16 mg g‑1 fresh weight (f.w.) with values ranging from 0.01- 4.06 mg g‑1 f.w.
Similarly, the average sugar content was 8.04 mg g‑1 f.w., with a range of 1.17 to 15.37 mg g‑1 f.w.
The metabolic diversity of the collection highlights a greater variability among the genotypes selected in the Mediterranean area compared to those with Asian and African origins.
The current rocket salad collection is being expanded to build a larger germplasm core-set for genome-wide association studies toward the dissection of the genetic basis of quality traits.
The findings and tools produced in this research represent a unique opportunity to revolutionize the breeding strategies in rocket salad.
Authors
P. Tripodi, R. DAlessandro, G. Festa, C. Di Cesare, I. Baiamonte, N. Nardo, A. Raffo
Keywords
Rad sequencing, vitamin C, sugars, genetic diversity, metabolic compounds, core collection
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