Articles
Could silicon ameliorate salt tolerance in Olea europaea L.?
Article number
1446_20
Pages
143 – 148
Language
English
Abstract
Olive trees are considered moderately tolerant to salinity, but tolerance mechanisms are cultivar and genotype dependent.
Silicon is a metalloid that has the potential to alleviate salt stress in plants.
The aim of this study is to evaluate the silicon priming effect on salt stress responses in salt-tolerant (‘Frantoio’) and salt-sensitive (‘Leccino’) cultivars.
One-year old self-rooted plants of both cultivars were irrigated for 28 days with 0.16 mM silicic acid H2SiO3 and then, for 51 days with 100 mM NaCl.
Photosynthetic performance decreased in both cultivars in response to NaCl; however, in ‘Frantoio’ this effect was exacerbated by silicon pre-treatment.
Ionomic profiling at whole plant level was conducted by atomic emission spectroscopy.
After 51 days of salt stress with or without silicon pre-treatment, sodium accumulation follows the order: roots > leaves, in both cultivars and in ‘Leccino’ pre-treated plants, silicon induces a reduction of 46% of sodium accumulation in new leaves.
Silicon had influence also on ion uptake and mobilization in both cultivars and in particular increases ion uptake (Fe, Mn) in new leaves of cultivar ‘Leccino’ and a decrease in cultivar ‘Frantoio’ under salt exposure.
Polyphenol concentrations in plants were determined by UHPLC-ESI-MS/MS mass spectrometry (Sciex 5500 QTrap+), using an information dependent acquisition (IDA) method.
Metabolomic analysis indicated that the synthesis of polyphenols that play a role in osmotic adjustment and antioxidative defence to cope with salt stress was affected.
Silicon is a metalloid that has the potential to alleviate salt stress in plants.
The aim of this study is to evaluate the silicon priming effect on salt stress responses in salt-tolerant (‘Frantoio’) and salt-sensitive (‘Leccino’) cultivars.
One-year old self-rooted plants of both cultivars were irrigated for 28 days with 0.16 mM silicic acid H2SiO3 and then, for 51 days with 100 mM NaCl.
Photosynthetic performance decreased in both cultivars in response to NaCl; however, in ‘Frantoio’ this effect was exacerbated by silicon pre-treatment.
Ionomic profiling at whole plant level was conducted by atomic emission spectroscopy.
After 51 days of salt stress with or without silicon pre-treatment, sodium accumulation follows the order: roots > leaves, in both cultivars and in ‘Leccino’ pre-treated plants, silicon induces a reduction of 46% of sodium accumulation in new leaves.
Silicon had influence also on ion uptake and mobilization in both cultivars and in particular increases ion uptake (Fe, Mn) in new leaves of cultivar ‘Leccino’ and a decrease in cultivar ‘Frantoio’ under salt exposure.
Polyphenol concentrations in plants were determined by UHPLC-ESI-MS/MS mass spectrometry (Sciex 5500 QTrap+), using an information dependent acquisition (IDA) method.
Metabolomic analysis indicated that the synthesis of polyphenols that play a role in osmotic adjustment and antioxidative defence to cope with salt stress was affected.
Publication
Authors
C. Fidalgo-Illesca, A. Francini, A. Raffaelli, L. Sebastiani
Keywords
ionomic, metabolomics, polyphenols, salinity, UHPLC-MS/MS analysis
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