Articles
Development of a deep proteomic pipeline for recalcitrant olive leaf tissue
Article number
1446_9
Pages
65 – 74
Language
English
Abstract
Proteomic analysis is a powerful tool to unravel the complexity of plant cellular processes that underpin the regulation of plant immunity.
A major challenge is the improvement of the detectable fraction of the crop proteome that is still markedly lower compared to other omics, such as next generation sequencing technologies.
This is due in part to the occurrence of large amounts of secondary compounds, which co-precipitate with proteins and severely interfere with the analysis.
Olive leaf tissue is notoriously recalcitrant to common protein extraction methods due to high levels of interfering compounds, hence hampering deep proteomic investigations.
Our study aims to overcome these hurdles and expand the application of deep proteomic analyses to olive leaves.
We developed a complete proteomic pipeline, from sample preparation to LC-HRMS and data analyses, allowing the first comparative proteomic study among three Italian olive cultivars, Leccino, Ogliarola and Coratina, known to exhibit different susceptibility to Xylella fastidiosa infections, and enabling the detection of 1922 proteins.
Olive proteomic research is expected to become an essential part of integrated omics approaches; thus, our study is a significant contribution, paving the way to unravel the molecular complexity underlying the genotype-dependent immune response to stress.
A major challenge is the improvement of the detectable fraction of the crop proteome that is still markedly lower compared to other omics, such as next generation sequencing technologies.
This is due in part to the occurrence of large amounts of secondary compounds, which co-precipitate with proteins and severely interfere with the analysis.
Olive leaf tissue is notoriously recalcitrant to common protein extraction methods due to high levels of interfering compounds, hence hampering deep proteomic investigations.
Our study aims to overcome these hurdles and expand the application of deep proteomic analyses to olive leaves.
We developed a complete proteomic pipeline, from sample preparation to LC-HRMS and data analyses, allowing the first comparative proteomic study among three Italian olive cultivars, Leccino, Ogliarola and Coratina, known to exhibit different susceptibility to Xylella fastidiosa infections, and enabling the detection of 1922 proteins.
Olive proteomic research is expected to become an essential part of integrated omics approaches; thus, our study is a significant contribution, paving the way to unravel the molecular complexity underlying the genotype-dependent immune response to stress.
Publication
Authors
R. Abbattista, P.A. Zaini, M. Salemi, B.S. Phinney, T.R.I. Cataldi, I. Losito, A.M. Dandekar
Keywords
crop proteomics, high-resolution mass spectrometry proteomics, protein extraction, olive leaf proteomics
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