Articles
First report of phytopathological problems during ginger postharvest season
Article number
1451_11
Pages
83 – 88
Language
English
Abstract
Peru is one of the main ginger exporting countries to USA, Canada, and Europe.
The export product is the rhizome, which is a vegetative structure that develops in the soil.
The objective of this research was to carry out a diagnosis of the health problems in the ginger postharvest handling.
During the years 2022 and 2023, samples of rhizomes with rot symptoms (from packinghouse discards) were taken.
A characterization of the different symptoms detected was made; according to the name assigned by the producers: “blackfoot”, “black spots”, “soft rot”, “silver”, “nodules”. Samples were washed with drinking water, then disinfested with 70% ethyl alcohol.
Sections of diseased tissue were plated in Petri dishes containing the culture medium PARB, PDA. The plates were incubated at 25°C in the dark for one week.
The following microorganisms were isolated: Rosselinia, Rhizoctonia solani, Pythium. The microorganisms were purified and increased for pathogenicity tests.
The inoculation method was done by pouring the purified microorganism on the base of ginger plants under semi-controlled greenhouse conditions.
Results were positive for Koch’s third postulate.
Molecular characterization of the isolates was done using the internal transcribed spacer region (ITS) and nuclear large subunit regions (LSU) of ribosomal DNA (rDNA). The following organisms were identified as ginger pathogens: Rhizoctonia solani, Fusarium oxysporum, Rosselinia bunnodes, Pythium splendens.
The export product is the rhizome, which is a vegetative structure that develops in the soil.
The objective of this research was to carry out a diagnosis of the health problems in the ginger postharvest handling.
During the years 2022 and 2023, samples of rhizomes with rot symptoms (from packinghouse discards) were taken.
A characterization of the different symptoms detected was made; according to the name assigned by the producers: “blackfoot”, “black spots”, “soft rot”, “silver”, “nodules”. Samples were washed with drinking water, then disinfested with 70% ethyl alcohol.
Sections of diseased tissue were plated in Petri dishes containing the culture medium PARB, PDA. The plates were incubated at 25°C in the dark for one week.
The following microorganisms were isolated: Rosselinia, Rhizoctonia solani, Pythium. The microorganisms were purified and increased for pathogenicity tests.
The inoculation method was done by pouring the purified microorganism on the base of ginger plants under semi-controlled greenhouse conditions.
Results were positive for Koch’s third postulate.
Molecular characterization of the isolates was done using the internal transcribed spacer region (ITS) and nuclear large subunit regions (LSU) of ribosomal DNA (rDNA). The following organisms were identified as ginger pathogens: Rhizoctonia solani, Fusarium oxysporum, Rosselinia bunnodes, Pythium splendens.
Authors
L. Aragón, E. Mendez, L. Dávila, M. Cerna, R. Acuña, A. Casas
Keywords
ginger postharvest rot, rot rhizomes
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