Articles
Indirect somatic embryogenesis of Moringa oleifera for genetic engineering
Article number
1460_13
Pages
103 – 110
Language
English
Abstract
Moringa oleifera (moringa) is a tropical plant that has high nutritional qualities and is used widely in feeding programs globally.
Native to India and other parts of Asia, moringa leaves, seeds, and roots have been used for their medicinal qualities.
The leaves and seeds are known for their antibacterial properties and are particularly effective against gram-positive bacteria. M. oleifera seeds are also used as a coagulant for freshwater purification.
A limitation of the moringa tree is its sensitivity to cold, limiting its ability to overwinter.
Improved cold tolerance of moringa is one way to improve this highly valuable and nutritious plant by increasing its capacity to grow outside of its native range.
Previous studies report methods for in vitro cloning moringa, and our study is the first to present a successful callus formation and regeneration method through embryogenesis.
Initiation of calli from moringa occurred using seed, on media consisting of 30 g L‑1 sucrose, half strength Murashige and Skoog with 0.1 mg L‑1 2,4-dichlorophenoxyacetic acid.
Calli were fully initiated within two to six weeks.
Regeneration of Moringa oleifera calli from excised embryos occurred on medium containing (L‑1) 30 g sucrose, 2.2 g Murashige and Skoog, 1.0 mg IAA, and 0.5 mg kinetin.
Native to India and other parts of Asia, moringa leaves, seeds, and roots have been used for their medicinal qualities.
The leaves and seeds are known for their antibacterial properties and are particularly effective against gram-positive bacteria. M. oleifera seeds are also used as a coagulant for freshwater purification.
A limitation of the moringa tree is its sensitivity to cold, limiting its ability to overwinter.
Improved cold tolerance of moringa is one way to improve this highly valuable and nutritious plant by increasing its capacity to grow outside of its native range.
Previous studies report methods for in vitro cloning moringa, and our study is the first to present a successful callus formation and regeneration method through embryogenesis.
Initiation of calli from moringa occurred using seed, on media consisting of 30 g L‑1 sucrose, half strength Murashige and Skoog with 0.1 mg L‑1 2,4-dichlorophenoxyacetic acid.
Calli were fully initiated within two to six weeks.
Regeneration of Moringa oleifera calli from excised embryos occurred on medium containing (L‑1) 30 g sucrose, 2.2 g Murashige and Skoog, 1.0 mg IAA, and 0.5 mg kinetin.
Authors
G.J. Miller, J.B. Noseworthy, J. Buck, R. Embalabala, H. Blanchard, C. Bennett, B. Russell, E. Holder
Keywords
Moringa oleifera, tissue culture, kinetin, auxin, medicinal plant
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