Articles
S-locus polymorphism of European cherry laurel cultivars
Article number
1467_24
Pages
203 – 208
Language
English
Abstract
Cherry laurel (Prunus laurocerasus L.) is an extreme polyploid ornamental species belonging to the Rosaceae family.
Our study aimed to identify the S-locus polymorphism in 12 European cherry laurel cultivars.
Characterization of S-alleles was revealed using PCR amplification of the first intron region in the S–ribonuclease (S–RNase). The evaluation of data was based on the fluorescently labeled (FAM) forward primer, and the amplicons were run on an automated capillary sequencer.
In 12 cultivars originating from different countries, a total of 47 alleles of varying sizes were detected.
This indicates a high degree of variability, which is associated with the extreme ploidy level.
In several cases, the difference between allele sizes was only a single nucleotide.
The total size range for the first intron was between 217 and 536 bp.
In a single individual, the highest observed allele number was 16, which confirms the previously supposed self-incompatible phenotype of P. laurocerasus. In the case of self-fertilization, a much higher accumulation of identical alleles would be expected; therefore, the detected allele numbers would likely have been lower.
This is the first study to describe the S-RNase alleles of ornamental cherry laurel cultivars.
Our study aimed to identify the S-locus polymorphism in 12 European cherry laurel cultivars.
Characterization of S-alleles was revealed using PCR amplification of the first intron region in the S–ribonuclease (S–RNase). The evaluation of data was based on the fluorescently labeled (FAM) forward primer, and the amplicons were run on an automated capillary sequencer.
In 12 cultivars originating from different countries, a total of 47 alleles of varying sizes were detected.
This indicates a high degree of variability, which is associated with the extreme ploidy level.
In several cases, the difference between allele sizes was only a single nucleotide.
The total size range for the first intron was between 217 and 536 bp.
In a single individual, the highest observed allele number was 16, which confirms the previously supposed self-incompatible phenotype of P. laurocerasus. In the case of self-fertilization, a much higher accumulation of identical alleles would be expected; therefore, the detected allele numbers would likely have been lower.
This is the first study to describe the S-RNase alleles of ornamental cherry laurel cultivars.
Authors
J. Halász, A. Hegedűs
Keywords
Prunus laurocerasus, S-alleles, S–RNase, self-incompatibility, docosaploid
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