Articles
DNA fingerprinting of the extreme polyploid Prunus laurocerasus cultivars by SSR analysis
Article number
1467_26
Pages
217 – 222
Language
English
Abstract
Breeding of the woody cherry laurel (Prunus laurocerasus L., Rosaceae family) has been the focus of many studies.
However, due to its extremely high ploidy level (2n=22x=176), the genetic background of the species remains largely unknown.
The application of molecular marker-based analyses could significantly facilitate breeding efforts.
A total of 12 ornamental cultivars (originated from Hungary, Bulgaria, France, Netherlands, Germany and Ireland) were characterized by 10 codominant microsatellite (SSR) markers.
After PCR amplification, fragment sizes were precisely determined using an ABI PRISM 3100 Genetic Analyzer, and data were processed with Peak Scanner 3.0. All loci were found to be polymorphic.
A total of 297 SSR alleles were identified.
The highest allele numbers were observed at the CPDCT044, BPPCT039, and BPPCT025 loci (52, 44, and 42 alleles, respectively), while the lowest number was detected at the trinucleotide repeat-containing ASSR63 locus (5 alleles). The overall allele size range across the 10 loci was between 99 and 280 bp, with the widest range observed at the CPDCT044 locus (118-280 bp). Since the allele number was high, the polymorphism of the analyzed loci enabled the differentiation of all genotypes, each characterized by a unique DNA fingerprint.
The analysis of allele frequencies indicated characteristic alterations among the major European cultivars.
This study represents an initial step toward a comprehensive genetic analysis of this highly polyploid species.
However, due to its extremely high ploidy level (2n=22x=176), the genetic background of the species remains largely unknown.
The application of molecular marker-based analyses could significantly facilitate breeding efforts.
A total of 12 ornamental cultivars (originated from Hungary, Bulgaria, France, Netherlands, Germany and Ireland) were characterized by 10 codominant microsatellite (SSR) markers.
After PCR amplification, fragment sizes were precisely determined using an ABI PRISM 3100 Genetic Analyzer, and data were processed with Peak Scanner 3.0. All loci were found to be polymorphic.
A total of 297 SSR alleles were identified.
The highest allele numbers were observed at the CPDCT044, BPPCT039, and BPPCT025 loci (52, 44, and 42 alleles, respectively), while the lowest number was detected at the trinucleotide repeat-containing ASSR63 locus (5 alleles). The overall allele size range across the 10 loci was between 99 and 280 bp, with the widest range observed at the CPDCT044 locus (118-280 bp). Since the allele number was high, the polymorphism of the analyzed loci enabled the differentiation of all genotypes, each characterized by a unique DNA fingerprint.
The analysis of allele frequencies indicated characteristic alterations among the major European cultivars.
This study represents an initial step toward a comprehensive genetic analysis of this highly polyploid species.
Authors
A. Hegedűs, J. Halász
Keywords
breeding, cherry laurel, docosaploid, genetic variability, microsatellite, simple sequence repeats
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