Articles
THE EFFECT OF OSMOTIC CONCENTRATION ON THE PRODUCTION OF HERBICOLACIN 112Y BY ERWINIA HERBICOLA 112Y
Article number
217_39
Pages
229 – 234
Language
Abstract
Erwinia herbicola strain Eh112Y sometimes produces a bacteriocion, called herbicolacin 112Y (H-112Y), that inhibits the growth of Erwinia amylovora, the fire blight pathogen.
Herbicolacin 112Y was not produced when Eh112Y was grown on a glucose-mineral salts medium with complex nitrogen sources.
Addition of nutrient broth, yeast extract, casamino acids, or a combination of nutrient broth and yeast extract inhibited production.
However, H-112Y was produced in the same media when the concentration of potassium phosphate was 0.18 M. Production of H-112Y was stimulated when the concentration of NaCl was 0.5 M or the concentration of sorbitol was 1.0 M. High osmotic concentration stimulated H-112Y production and did not simply cause the release of H-112Y that adhered to cell components.
Herbicolacin 112Y was not produced when Eh112Y was grown on a glucose-mineral salts medium with complex nitrogen sources.
Addition of nutrient broth, yeast extract, casamino acids, or a combination of nutrient broth and yeast extract inhibited production.
However, H-112Y was produced in the same media when the concentration of potassium phosphate was 0.18 M. Production of H-112Y was stimulated when the concentration of NaCl was 0.5 M or the concentration of sorbitol was 1.0 M. High osmotic concentration stimulated H-112Y production and did not simply cause the release of H-112Y that adhered to cell components.
Publication
Authors
R.S. Wodzinski, G. Stegner, S.V. Beer, P. Sobiczewski
Keywords
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