EFFECTS OF BASAL MEDIUM COMPOSITION ON THE GROWTH OF CARNATION PLANTLETS IN AUTO- AND MIXO-TROPHIC TISSUE CULTURE

Explants of carnation (Dianthus caryophyllus L.) were cultured in vitro, using air-leaky vessels, under high photosynthetic photon flux on either sugar-free or sugar-containing media containing either a half strength Murashige and Skoog (1962) basal composition or a full-strength Enshi (Hori, 1966) basal composition of nutrient solution for hydroponics.

Then, time courses of fresh and dry weights of the plantlets, CO₂ concentration in the vessel were measured.
Furthermore, the net photosynthetic rate, dark respiration rate and the relative growth rate of the plantlet were estimated.

The order of total fresh weight per plantlet on the 30th day after the start of the experiment from largest to smallest is, 1) no sugar and the Enshi basal composition treatment, 2) 2% sucrose and the Enshi basal composition treatment, 3) no sugar and the ½ MS basal composition treatment, and 4) 2% sucrose and the ½ MS basal composition treatment.

Under high photosynthetic photon flux, the growth of the plantlet in the air-leaky vessels are considered to be more promoted on the sugar-free medium with the Enshi basal composition than on the sugar-containing medium with the ½ MS or Enshi basal composition, or the sugar-free medium with the ½ MS basal composition.

Under the autotrophic or heterotrophic growth conditions, the favorable basal compositions for growth seemed to be different from those developed for the heterotrophic growth of tissue or organ, such as Murashige and Skoog (1962) medium.
Implications of the results obtained are discussed based on a primitive growth analysis.

Symposium on High Technology in Protected Cultivation

T. Kozai, C. Kubota, I. Watanabe

https://doi.org/10.17660/ActaHortic.1988.230.17