Articles
PLANTLET REGENERATION FROM PROTOPLASTS OF KIWIFRUIT, ACTINIDIA CHINENSIS PLANCH
Article number
230_18
Pages
167 – 170
Language
Abstract
Protoplasts isolated from the cell suspension culture of Actinidia chinensis were cultured in the modified MS medium containing 1×10-6M each of BA and 2,4-D and 0.5M mannitol.
The plating efficiency after two weeks of culture was about 40%. However, for further growth of the colonies, dilution of osmoticum concentration in the medium was required.
Shoot regeneration from the protoplast-derived calli was observed only when the calli were transferred onto the regeneration medium after two passages of subculture on the preconditioning medium (MS medium supplemented with NAA and BA) or when the regeneration medium was replaced by the fresh one after on passage of subculture on the preconditioning medium.
No plantlet regeneration occurred without applying preconditioning medium or changing regeneration medium to the fresh one.
The plating efficiency after two weeks of culture was about 40%. However, for further growth of the colonies, dilution of osmoticum concentration in the medium was required.
Shoot regeneration from the protoplast-derived calli was observed only when the calli were transferred onto the regeneration medium after two passages of subculture on the preconditioning medium (MS medium supplemented with NAA and BA) or when the regeneration medium was replaced by the fresh one after on passage of subculture on the preconditioning medium.
No plantlet regeneration occurred without applying preconditioning medium or changing regeneration medium to the fresh one.
The shoots thus obtained were easily rooted on the ½ MS medium lacking growth regulators and successfully established on the soil.
Authors
M. Mii, H. Ohashi
Keywords
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