Articles
SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN THE TISSUE CULTURE OF GEONOMA GAMIOVA (ARECACEAE)
Article number
360_21
Pages
167 – 172
Language
Abstract
The severe devastation taking place in the Atlantic Forest of Brazil is threatening Geonoma species.
These species are easily propagated by seeds, but it is necessary to develop techniques of vegetative propagation such as tissue culture to guarantee fidelity of clones.
Immature zygotic embryos originating from young fruits of G. gamiova were inoculated in semi-solid culture media in the absence of light.
The media were constituted by the MS salts, Morel vitamins, and complemented with 30 g/l of sucrose, 1.5 g/l of activated charcoal, 100 g/l of 2,4-D and 3 mg/l of 2iP. At the 30th day after inoculation, it was observed a formation of embryogenetic masses from the epidermic tissue of the mesocotyl.
The isolation and culture of these cellular masses in secondary media constituted of the same basal formulation with a lower concentration of 2,4-D (20 g/l) allowed the mass regeneration of these pro-embryogenetic cells.
The transferring of this cell masses to culture media without growth regulators allowed the progress to late stages of embryogenesis and the conversion to plantlets.
Alternatively, bipolar somatic embryos were encapsulated in sodium alginate originating synthetic seeds.
The protocol generated allows the mass propagation from superior genotypes of G. gamiova.
These species are easily propagated by seeds, but it is necessary to develop techniques of vegetative propagation such as tissue culture to guarantee fidelity of clones.
Immature zygotic embryos originating from young fruits of G. gamiova were inoculated in semi-solid culture media in the absence of light.
The media were constituted by the MS salts, Morel vitamins, and complemented with 30 g/l of sucrose, 1.5 g/l of activated charcoal, 100 g/l of 2,4-D and 3 mg/l of 2iP. At the 30th day after inoculation, it was observed a formation of embryogenetic masses from the epidermic tissue of the mesocotyl.
The isolation and culture of these cellular masses in secondary media constituted of the same basal formulation with a lower concentration of 2,4-D (20 g/l) allowed the mass regeneration of these pro-embryogenetic cells.
The transferring of this cell masses to culture media without growth regulators allowed the progress to late stages of embryogenesis and the conversion to plantlets.
Alternatively, bipolar somatic embryos were encapsulated in sodium alginate originating synthetic seeds.
The protocol generated allows the mass propagation from superior genotypes of G. gamiova.
Publication
Authors
A.C. Dias, M.P. Guerra, A.S. Cordoba, E.L. Kemper
Keywords
asexual propagation, in vitro culture, germplasm conservation
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