Articles
USE OF A FLOW-CYTOMETER FOR POLLEN COUNTING – AN APPLICATION TO VICIA FABA L.
Article number
437_46
Pages
369 – 372
Language
Abstract
The accuracy of flow-cytometry for measuring pollen production by flowers was tested in a preliminary experiment conducted with Vicia faba. Thirty eight samples ranging from 200 to 3,950 grains, were deposited on slides and photographed under a microscope.
Each sample was washed out into a tube and counted through a cytometer "Partec II". Counts by the microscopic photograph technique, and the cytometer, were closely correlated (r2 = 0.998). Cytometry was thus used to compare pollen removal from flowers of V. faba visited by Bombus pascuorum. The number of grains removed by pollen-collecting workers from flowers visited once or twice was 24.3 and 48.7% respectively of the initial average amount of 29,600 ± 800 (x ± se). Visits by nectar-collecting queens resulted in similar removals (35.2 and 45.9%).
Each sample was washed out into a tube and counted through a cytometer "Partec II". Counts by the microscopic photograph technique, and the cytometer, were closely correlated (r2 = 0.998). Cytometry was thus used to compare pollen removal from flowers of V. faba visited by Bombus pascuorum. The number of grains removed by pollen-collecting workers from flowers visited once or twice was 24.3 and 48.7% respectively of the initial average amount of 29,600 ± 800 (x ± se). Visits by nectar-collecting queens resulted in similar removals (35.2 and 45.9%).
Publication
Authors
S. Carré, J.N. Taséi
Keywords
Online Articles (62)