Articles
ETHYLENE-RESPONSIVE GENE EXPRESSION DURING CARNATION FLOWER SENESCENCE
Article number
464_17
Pages
135 – 140
Language
Abstract
Ethylene activates the transcription of a number of genes at the onset of senescence in carnation flower petals.
A glutathione S-transferase gene (GST1) has been used as a model to study the mechanism by which ethylene induces gene expression.
A 126 bp region of the GST1 promoter sequence has been identified as an ethylene-responsive enhancer element (ERE). Nuclear proteins from senescing petals recognize a 22 bp sequence within the ERE, and mutations in this sequence disrupt the interaction between the protein and DNA. The wild-type ERE sequence was used to isolate a cDNA encoding a sequence-specific DNA binding protein.
Nucleotide sequencing and deduced amino acid sequence analysis of this cDNA predicted a 32 kDa protein that we have designated CEBP-1. The mRNA expression pattern of CEBP-1 suggests that it is not transcriptionally regulated by ethylene.
The amino acid sequence homology of CEBP-1 with other plant nucleic acid binding proteins indicates a conserved nucleic acid binding domain.
Within this domain are two highly conserved RNA-binding motifs.
Taken together, these data suggest that CEBP-1 represents a component of a DNA-binding complex involved in the transcriptional activation of GST1 by ethylene.
A glutathione S-transferase gene (GST1) has been used as a model to study the mechanism by which ethylene induces gene expression.
A 126 bp region of the GST1 promoter sequence has been identified as an ethylene-responsive enhancer element (ERE). Nuclear proteins from senescing petals recognize a 22 bp sequence within the ERE, and mutations in this sequence disrupt the interaction between the protein and DNA. The wild-type ERE sequence was used to isolate a cDNA encoding a sequence-specific DNA binding protein.
Nucleotide sequencing and deduced amino acid sequence analysis of this cDNA predicted a 32 kDa protein that we have designated CEBP-1. The mRNA expression pattern of CEBP-1 suggests that it is not transcriptionally regulated by ethylene.
The amino acid sequence homology of CEBP-1 with other plant nucleic acid binding proteins indicates a conserved nucleic acid binding domain.
Within this domain are two highly conserved RNA-binding motifs.
Taken together, these data suggest that CEBP-1 represents a component of a DNA-binding complex involved in the transcriptional activation of GST1 by ethylene.
Authors
J.M. Maxson, W.R. Woodson
Keywords
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