Home > IX International Asparagus Symposium > OPTIMIZING THE DELIVERY OF DNA AND TRANSIENT GENE EXPRESSION IN ASPARAGUS CELLS BY BIOLISTIC BOMBARDMENT

Articles

OPTIMIZING THE DELIVERY OF DNA AND TRANSIENT GENE EXPRESSION IN ASPARAGUS CELLS BY BIOLISTIC BOMBARDMENT

Article number

479_46

Pages

335 – 338

Language

Abstract

The delivery of DNA by biolistic bombardment to asparagus cells and its expression were found to be affected by the rupture disc, microcarrier, osmotic potential, promoter of the gene, and the cell genotype.
Significant improvement could by obtained by optimizing these factors.

Publication

IX International Asparagus Symposium

Authors

A. Chackalamannil, J. Eberhard, C. Laramore, F. Belanger, C. Chin, S. Garrison

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1999.479.46

Online Articles (65)

2. LINKING MARKETING STRATEGIES WITH RESEARCH

L. McKeown

3. WORLD ASPARAGUS PRODUCTION AREAS AND PERIODS OF PRODUCTION

B.L. Benson

4. RECENT PRODUCTION AND IMPORT OF ASPARAGUS IN JAPAN

H. Araki

5. ASPARAGUS IN THE PERUVIAN COASTAL REGION: PRESENT AND FUTURE

J. Sánchez | A. Casas

6. ACTION NEEDED ON ASPARAGUS GERMPLASM CONSERVATION: A WORKSHOP

W.A. Jermyn | R.J. Cross

7. ACHIEVEMENT OF ASPARAGUS BREEDING IN ITALY

A. Falavigna | P.E. Casali | A. Battaglia

8. CONSTRUCTION OF A LINKAGE MAP OF ASPARAGUS OFFICINALIS THROUGH DIFFERENT MOLECULAR MARKERS

9. ISOZYME GENE MARKERS IN ASPARAGUS USED TO CLASSIFY FIFTY ASPARAGUS CULTIVARS

M.L. González-Castañón

10. CLADOPHYLL CHARACTERISTICS AS POSSIBLE CONTRIBUTORS TO GENETIC VARIATION IN ASPARAGUS FERN PHOTOSYNTHETIC CAPACITY

M.J. Faville | T.G.A. Green | W.B. Silvester | W.A. Jermyn

11. GENETIC VARIATION FOR TIPROT IN ASPARAGUS

W.A. Jermyn | R.E. Lill | M. Ruhen

12. GENETIC VARIATION IN THE RATE OF ASPARAGUS FERN PHOTOSYNTHESIS

M.J. Faville | T.G.A. Green | W.B. Silvester | W.A. Jermyn

13. GENETICS AND BREEDING OF ASPARAGUS AT THE UNIVERSITY OF CALIFORNIA, RIVERSIDE

Mikeal L. Roose | Neil K. Stone

14. BREEDING PURPLE ASPARAGUS FROM TETRAPLOID “VIOLETTO D’ALBENGA”

Peter G. Falloon | A.M. Andersen

15. HERBICIDE RESISTANCE IN ASPARAGUS: IN VITRO CELL SELECTION AND CHARACTERIZATION

D. Ganeshan | A.J. Conner | A.G. Fautrier

16. DETECTION OF VARIATION WITHIN ASPARAGUS EMBRYOGENIC CALLI USING RAPD MARKERS

W.O. Hollingsworth | C.B. Christie | M.A. Nichols | M.H. Behboudian

17. MOLECULAR MARKERS LINKED TO THE SEX DETERMINATION LOCUS OF ASPARAGUS

Y. Ozaki | T. Tashiro | H. Okubo

18. EVALUATION OF ASPARAGUS OFFICINALIS L. FLAVOUR QUALITY FOR BREEDING PURPOSES

E. Hoberg | D. Ulrich | D. Standhardt | S. Kecke

19. SECOND INTERNATIONAL ASPARAGUS CULTIVAR TRIAL

B.L. Benson

20. PRELIMINARY RESULTS OF THE SECOND INTERNATIONAL ASPARAGUS CULTIVAR TRIAL IN ICA, PERU

E. Oordt | F. Vaccari | J. Carillo | P. Velásquez | W. Apaza

21. IMPROVING THE EFFICIENCY OF CULTIVAR EVALUATION OF GREEN ASPARAGUS

M. A. Nichols | Keith J. Fisher

22. YIELD PERFORMANCE OF NEW ASPARAGUS CULTIVARS AT DOLE TROPIFRESH, POLOMOLOK, PHILIPPINES

G.G. Cueto | D.J. Lesnick

23. VEGETATIVE GROWTH AND SPEAR YIELD OF EXOTIC ASPARAGUS CULTIVARS GROWN IN UGANDA

E. Bakka | R. Ssonko | V. Makumbi | B.P. Singh

24. ASPARAGUS CULTIVAR EVALUATION TRIALS IN THE NORTHERN SAN JOAQUIN VALLEY OF CALIFORNIA

Robert J. Mullen | T.C. Viss | R.S. Whitely

25. PERFORMANCE OF CULTIVARS AND LINES OF ASPARAGUS IN MICHIGAN AND NEW JERSEY

S. A. Garrison | C. Chin | J. Bakker | John F. Kelly

26. FIELD EVALUATION OF NEW ASPARAGUS VARIETIES AT THE UNIVERSITY OF CALIFORNIA, RIVERSIDE

Neil K. Stone | Mikeal L. Roose

27. ASPARAGUS CULTIVAR TRIALS IN CONNECTICUT

W.H. Elmer | J.A. LaMondia | G.S. Taylor

28. EFFECTIVENESS OF CLIMATE, CULTIVAR, AND FUNGICIDE IN CONTROLLING RUST IN ASPARAGUS

K. Ogwal | E. Adipala | S.A.P. Owera | B.P. Singh

29. EPIDEMIOLOGY OF STEMPHYLIUM LEAF SPOT AND PURPLE SPOT IN NO-TILL ASPARAGUS

M.K. Hausbeck | J. Hartwell | J.M. Byrne

30. STUDIES ON THE SUPPRESSION OF FUSARIUM CROWN AND ROOT ROT OF ASPARAGUS WITH NACL

W.H. Elmer | J.A. LaMondia

31. CHARACTERIZATION OF FUSARIUM POPULATIONS IN ASPARAGUS FIELDS IN THE PACIFIC NORTHWEST

M.C. Doan | L.M. Carris

32. FUSARIUM OXYSPORUM PROLIFERATUM AND FUSARIUM OXYSPORUM INFECTION OF COMMERCIAL ASPARAGUS SPEARS ORIGINATES FROM INFECTED CROWNS IN THE FIELD

C. Guerrero | E.L. Nigh Jr | M.E. Stanghellini

33. INCIDENCE OF FUSARIUM SPP. IN ASPARAGUS FIELDS IN MEXICO AND SOUTHERN CALIFORNIA

C. Guerrero | E.L. Nigh Jr | M.E. Stanghellini

34. A BIOASSAY TO MONITOR THE AUTOTOXIN LEVELS IN ASPARAGUS REPLANT SOILS

P.E. Schofield | M.A. Nichols | P.G. Long | B.R. MacKay

35. EVALUATION OF FUSARIUM SPP. INFECTED ASPARAGUS SPEARS FOR FUMONISIN MYCOTOXINS

Edward L. Nigh Jr | C. Guerrero | M.E. Stanghellini

36. ASPARAGUS DECLINE AND REPLANT PROBLEM: A REVIEW OF THE CURRENT SITUATION AND APPROACHES FOR FUTURE RESEARCH

P.C. Keulder

37. ASPARAGUS VIRUS 2: A CONTRIBUTING FACTOR IN ASPARAGUS DECLINE

M. V. Jaspers | Peter G. Falloon

38. EVALUATION OF ASPARAGUS SPEARS INFECTED WITH FUSARIUM SPECIES ON SHELF-LIFE AND QUALITY

Edward L. Nigh Jr | C. Guerrero | M.E. Stanghellini

39. CYTOTOXIC ACTIVITY OF STEROIDAL SAPONINS FROM ASPARAGUS OFFICINALIS

40. IRRIGATION EFFECTS ON ASPARAGUS ROOT DISTRIBUTION

D.T. Drost

41. THE EFFECT OF TEMPERATURE ON ASPARAGUS SPEAR GROWTH AND CORRELATION OF HEAT UNITS ACCUMULATED IN THE FIELD WITH SPEAR YIELD

Bill B. Dean

42. MODEL OF THE INFLUENCE OF TEMPERATURE ON THE ELONGATION RATE OF ASPARAGUS SPEARS

D.R. Wilson | C.G. Cloughley | S.M. Sinton

43. CARBOHYDRATE STORAGE AND RE-MOBILIZATION IN ASPARAGUS: STUDIES USING DRY WEIGHT CHANGES, C-14 AND HIGH PRESSURE LIQUID CHROMATOGRAPHY

D.J. Woolley | A.R. Hughes | M.A. Nichols

44. INFLUENCE OF TIME OF SPEAR HARVEST ON ROOT SYSTEM RESOURCES DURING THE ANNUAL GROWTH CYCLE OF ASPARAGUS

D.R. Wilson | S.M. Sinton | C.E. Wright

45. EARLY FLOWERING PROTEIN IN THE ARTIFICIAL FLOWERING PHASE IN ASPARAGUS SEEDLINGS

T. Abe | D. Yeo | H. Abe | S. Yoshida

46. DETECTION OF EXTRACELLULAR PROTEINS SECRETED BY ASPARAGUS EMBRYOGENIC CELLS IN SUSPENSION CULTURES

W.O. Hollingsworth | C.B. Christie | M.A. Nichols | M.H. Behboudian

47. OPTIMIZING THE DELIVERY OF DNA AND TRANSIENT GENE EXPRESSION IN ASPARAGUS CELLS BY BIOLISTIC BOMBARDMENT

48. TRANSIENT β-GLUCURONIDASE EXPRESSION IN ASPARAGUS PROTOPLASTS FOLLOWING ELECTROPORATION

C. Guangyu | A.J. Conner | A.G. Fautrier | R.J. Field

49. COMPARATIVE PERFORMANCE OF MALE AND FEMALE PLANTS DURING THE ANNUAL GROWTH CYCLE OF A DIOECIOUS ASPARAGUS CULTIVAR

S.M. Sinton | D.R. Wilson

50. DEVELOPMENT OF A MICROSPORE CULTURE METHOD TO PRODUCE HAPLOID AND DOUBLED-HAPLOID ASPARAGUS (ASPARAGUS OFFICINALIS L.) PLANTS

M. Peng | D.J. Wolyn

51. CLONING AND VARIATION OF RIBOSOMAL DNA FROM ASPARAGUS OFFICINALIS L.

A. Kanno | T. Kameya

52. AN EFFICIENT MICROPROPAGATION SYSTEM USING DISINFECTANT INCORPORATED MEDIUM AND FILM CULTURE VESSEL FOR IN VITRO PLANT REGENERATION OF ASPARAGUS

H. Kohmura | T. Yanagawa | M. Tanaka

53. SOIL WATER DEFICITS REDUCE GROWTH AND YIELD OF ASPARAGUS

D.T. Drost

54. TIMING OF ASPARAGUS PRODUCTION IN A TEMPERATE CLIMATE

M. Knaflewski | P. Kucharski | W. Krzesinski

55. SHORT TERM CROPPING OF ASPARAGUS

Heru D. Wardana | Keith J. Fisher | M. A. Nichols

56. COMMERCIAL PRODUCTION OF HIGH QUALITY HYBRID ASPARAGUS SEED WITH HIGH GENETIC PURITY

S. Walker | Lon K. Inaba | S. A. Garrison | C. Chin | W. Odermott

57. PLANT SPACING EFFECTS ON YIELD AND SIZE OF ASPARAGUS

John F. Kelly | J. Bakker | Hugh C. Price | Norman L. Myers

58. NITROGEN-POTASSIUM INTERACTIONS IN ASPARAGUS

D.C. Sanders

59. PRE-SENESCENCE REMOVAL OF ASPARAGUS (ASPARAGUS OFFICINALIS L.) FERN

John F. Kelly | Y. Bai

60. STUDIES WITH THE ASPARAGUS ‘MOTHER FERN’ CULTURE IN A TEMPERATE CLIMATE

I.L. Lekholoane | M.A. Nichols | K.J. Fisher

61. INFLUENCE OF CATCH CROPS ON LEACHING, YIELD AND QUALITY OF WHITE ASPARAGUS (ASPARAGUS OFFICINALIS L.)

P.-J. Paschold | B. Artelt | G. Hermann

62. EFFECTS OF MECHANIZED HARVESTING ON REVENUES FROM PROCESSED ASPARAGUS

R. J. Hopper | R. J. Folwell

63. WINTER TILLAGE SYSTEMS AND THEIR EFFECT ON ASPARAGUS YIELD AND WEED POPULATION

M.I. González | A. France | A. Del Pozo | A. Pedreros | V. Kramm

64. EFFECTS OF HARVEST AND POST-HARVEST TREATMENTS ON REDUCTION OF RED DISCOLOURATION IN WHITE ASPARAGUS

J.T.K. Poll | W. van den Berg

65. BLANCHING TO ACTIVATE PECTIN METHYLESTERASE DOES NOT IMPROVE PROCESSED ASPARAGUS TEXTURE

D. Burton | J.R. Powers