Articles
CONSTRUCTION OF A LINKAGE MAP OF ASPARAGUS OFFICINALIS THROUGH DIFFERENT MOLECULAR MARKERS
Article number
479_7
Pages
75 – 76
Language
Abstract
Genetic maps are useful tools for plant breeding: they represent sources of markers to locate single or quantitative trait loci and can be used in marker-facilitated breeding programs.
In a previous work we reported a linkage arrangement of RFLP loci in progenies from crosses between doubled haploid asparagus clones (Restivo et al., Theor.
Appl.
Genet. 90: 124–128, 1995) and more recently, a linkage map with 48 RFLP markers arranged in 14 groups was reported (Lewis and Sink.
Genome 39: 622–627, 1996). However, a saturated map of asparagus is not yet available.
In this work we present a linkage arrangement derived from the integration of the RFLP markers with two other molecular markers, RAPD and AFLP. The AFLP technique which has recently been adopted has already provided a high percent of polymorphism and several markers which have been integrated in the map.
The construction of an asparagus map allows the identification of sex-linked markers; in the previous work we reported three RFLP markers of this kind; in this work we show two more RFLP and one more AFLP marker which segregates with sex.
A saturated map of the sex chromosome could in the future allow the isolation of sex determining genes by positional cloning.
In a previous work we reported a linkage arrangement of RFLP loci in progenies from crosses between doubled haploid asparagus clones (Restivo et al., Theor.
Appl.
Genet. 90: 124–128, 1995) and more recently, a linkage map with 48 RFLP markers arranged in 14 groups was reported (Lewis and Sink.
Genome 39: 622–627, 1996). However, a saturated map of asparagus is not yet available.
In this work we present a linkage arrangement derived from the integration of the RFLP markers with two other molecular markers, RAPD and AFLP. The AFLP technique which has recently been adopted has already provided a high percent of polymorphism and several markers which have been integrated in the map.
The construction of an asparagus map allows the identification of sex-linked markers; in the previous work we reported three RFLP markers of this kind; in this work we show two more RFLP and one more AFLP marker which segregates with sex.
A saturated map of the sex chromosome could in the future allow the isolation of sex determining genes by positional cloning.
Publication
Authors
E. Caporali, P. Portaluppi, A. Spada, G. Marziani, A. Falavigna, F. Restivo, F. Tassi
Keywords
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