Articles
ISOZYME GENE MARKERS IN ASPARAGUS USED TO CLASSIFY FIFTY ASPARAGUS CULTIVARS
Article number
479_8
Pages
77 – 84
Language
Abstract
Asparagus cultivars are difficult to identify because many differ only in characters that are influenced by environmental conditions.
Isozyme markers were used to classify hybrids and cultivars.
Three of them, malate dehydrogenase (MDH), phosphoglucomutase (PGM) and shikimate dehydrogenase (SKD) showed inter- and intra- cultivar polymorphism Fifty cultivars coming from several countries: 12 from the USA; 6 from the Netherlands, 7 from France, 9 from Spain, 3 from Italy, 6 from Germany, 2 from New Zealand, 1 from Denmark, 1 from Japan and 3 from Taiwan were analyzed by starch gel electrophoresis for isoenzyme polymorphism.
Nine polymorphic bands in MDH, four in SKD, and nine in PGM were described.
It was possible to classify the fifty cultivars into seven groups by the patterns obtained with SKD. Each of these groups was further separated by the different patterns obtained with MDH. Using the different patterns obtained with SKD and MDH it was possible to group the fifty cultivars into 33 different classes.
The cultivars coming from the homozygous parental showed only a pattern in each of the isoenzymes studied.
Isozyme markers were used to classify hybrids and cultivars.
Three of them, malate dehydrogenase (MDH), phosphoglucomutase (PGM) and shikimate dehydrogenase (SKD) showed inter- and intra- cultivar polymorphism Fifty cultivars coming from several countries: 12 from the USA; 6 from the Netherlands, 7 from France, 9 from Spain, 3 from Italy, 6 from Germany, 2 from New Zealand, 1 from Denmark, 1 from Japan and 3 from Taiwan were analyzed by starch gel electrophoresis for isoenzyme polymorphism.
Nine polymorphic bands in MDH, four in SKD, and nine in PGM were described.
It was possible to classify the fifty cultivars into seven groups by the patterns obtained with SKD. Each of these groups was further separated by the different patterns obtained with MDH. Using the different patterns obtained with SKD and MDH it was possible to group the fifty cultivars into 33 different classes.
The cultivars coming from the homozygous parental showed only a pattern in each of the isoenzymes studied.
Publication
Authors
M.L. González-Castañón
Keywords
gene markers, asparagus classification
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