Home > II International Symposium on Chestnut > PRODUCTION OF PROBES FOR THE CHESTNUT GENOTYPES BY RAPD MARKERS

Articles

PRODUCTION OF PROBES FOR THE CHESTNUT GENOTYPES BY RAPD MARKERS

Article number

494_43

Pages

287 – 294

Language

Abstract

The rapid generation of probes for several chestnut cultivars by the RAPD technique is a method that does not require any preliminary information on the genome of the species to be investigated.
The procedure is based on the production of RAPD fingerprinting.
The amplification product(s) peculiar to one cultivar or common to two or more types of chestnut can be purified and used as molecular probes in hybridisation experiments for the identification of cultivars.

We tested this methodology on chestnut genotypes by amplifying the total DNA extracted from several chestnut cultivars.

Publication

II International Symposium on Chestnut

Authors

D. Paffetti, E. Bellini, R. Giannini

Keywords

Castanea sativa, molecular marker, cultivar, characterisation

Full text

https://doi.org/10.17660/ActaHortic.1999.494.43

Online Articles (78)

1. HIGHLIGHTS OF CHESTNUT INDUSTRY IN FRANCE

H. Breisch

2. THE SWEET CHESTNUT THROUGHOUT HISTORY FROM THE MIOCENE TO THE THIRD MILLENNIUM

M. Adua

3. CHESTNUT PRODUCTION IN CHILE. SOME STEPS TOWARD ITS IMPROVEMENT

P. Grau | A. France

4. CHESTNUT PRODUCTION AND CHESTNUT RESEARCH IN NEW ZEALAND

D. Klinac | R. Seelye | R. Knowles | H. Nguyen

5. SWEET CHESTNUT PRODUCTION AND MARKETING IN ITALY

M. Adua

6. MARKET DEVELOPMENT OPPORTUNITIES IN THE AUSTRALIAN CHESTNUT INDUSTRY

J.D. Ridley

7. A READY-TO-EAT CHESTNUT FLOUR BASED BREAKFAST CEREAL. PRODUCTION AND OPTIMIZATION.

G. Sacchetti | G.G. Pinnavaia

8. CHESTNUT TREES AND THE LANDSCAPE IN AOSTA VALLEY: INTERVENTIONS FOR RESTORING THE TRADITIONAL AGRICULTURAL SYSTEM

G. Bounous | A. Barrel | F. Pollano | D. Gyppaz

9. THE LANDSCAPE VALUE OF CHESTNUT GROVE IN CUNEO PROVINCE (ITALY)

G. Bounous | R. Paglietta | C. Peano | G. Giacalone | F. Parola

10. EVALUATION OF SOME CHESTNUT SELECTIONS FROM THE POPULATION FORMED INTO THE ECOLOGICAL CONDITIONS FROM THE NORTH – EAST OF OLTENIA

M. Botu | Gh. Achim | E. Turcu

11. SOME CONSIDERATIONS ON CHESTNUT DEVELOPMENT IN THE 21ST CENTURY IN CHINA

L. Liu | J.Y. Zhou

12. CHESTNUT WOODS ON MADONIE MOUNTAINS (SICILY, ITALY): REASONS FOR ABANDONMENT AND POSSIBILITIES OF RECOVERY

L. Gherardi | T. La Mantia | D.S. La Mela Veca

13. CHARACTERISATION OF SEVEN PRESELECTED CHESTNUT FRUIT TYPES FROM SLOVENE CHESTNUT (CASTANEA SATIVA MILL.) POPULATION

A. Solar | F. Štampar | A. Podjavoršek | A. Šiftar | I. Kodric

14. CHESTNUT HARVESTER FOR MOUNTAINOUS AREAS

R. Berruto | G. Ghiotti

15. TECHNOLOGICAL PROPERTIES OF CHESTNUT VARIETIES GROWN IN ERFELEK DISTRICT OF SINOP CITY

N. Üstün | Y. Tosun | Ü. Serdar

16. STUDY ON THE PRODUCT FEASIBILITY OF PRESERVED CHESTNUTS UNDER LOW SUGAR CONTENT SYRUP

G.G. Pinnavaia | G. Sacchetti | C. Chaves-Lopez | S. Romani

17. QUALITY ASSESSMENT OF CHESTNUT FRUITS

18. MORPHOLOGICAL VARIATION IN CHESTNUT FRUITS (CASTANEA SATIVA MILL.) IN SLOVENIA

A. Podjavoršek | F. Štampar | A. Solar | F. Batic

19. LIPID COMPOSITION OF CASTANEA SATIVA MILL. FRUITS OF SOME NATIVE PORTUGUESE CULTIVARS

J.V. Ferreira-Cardoso | C.A. Sequeira | J.M.G. Torres-Pereira | L. Rodrigues | E.F. Gomes

20. CHESTNUT ENVELOPES CONTAIN XYLAN- AND POLYGALACTURONIC ACID-RICH POLYSACCHARIDES

V. Gloaguen | D. Nourani | H. Morvan

21. EFFECT OF INK DISEASE ON CHESTNUT LITTERFALL PRODUCTION AND ON NUTRIENT BUDGETS OVER A 3-YEAR PERIOD

22. EFFECT OF LEAF TO FRUIT RATIOS ON FRUIT GROWTH IN CHESTNUT

F. Famiani | P. Proietti | A. Palliotti | E. Antognozzi | F. Ferranti

23. DYNAMICS OF LEAF LITTER STRUCTURAL COMPOUNDS IN C. SATIVA AND P. PINASTER FOREST ECOSYSTEMS DURING THE DECOMPOSITION PROCESS: INTERACTIONS WITH SOIL ORGANIC MATTER AND NUTRIENT RELEASE

A. Martins | S. Azevedo | L. Carvalho

24. PECTIC STATUS AND CALCIUM NUTRITION IN MICROPROPAGATED CHESTNUT SHOOTS

V. Freyssac | F. Marga | A. Laroche | H. Morvan

25. LEAF CHLOROPHYLL PARAMETERS FROM NORTH- AND SOUTH-EXPOSED CANOPY SECTORS OF CHESTNUT (CASTANEA SATIVA MILL.) CV. LONGAL: RELATION TO LEAF AND FRUIT SIZES

J.C.E. Gomes-Laranjo | V.M.F. Galhano | M.S.B.C. Torres-Pereira | J.M.G. Torres-Pereira

26. INFLUENCE OF LEAF POSITION, FRUIT AND LIGHT AVAILABILITY ON PHOTOSYNTHESIS IN DIFFERENT CHESTNUT GENOTYPES

27. EFFECT OF SXC^* ON RESPIRATORY RATE, PHOTOSYNTHESIS AND CHLOROPHYLL CONTENT OF CHINESE CHESTNUT LEAVES

Z. Zhao | K. Liu

28. EFFECT OF THINNING CATKINS ON NUTRITION IN THE CHINESE CHESTNUT TREE

K. Liu | Z. Zhao | C. Li

29. MINERAL AND PECTIC CONTENTS OF YOUNG CHESTNUT TREES GROWN UNDER CONTROLLED NUTRITION CONDITIONS

A. Laroche | V. Freyssac | J.P. Verger | H. Morvan

30. EVOLUTION OF AMANITA CAESAREA (SCOP.: FR.) PERS. AND BOLETUS EDULIS BULL.: FR. SYNTHETIC ECTOMYCORRHIZAE ON EUROPEAN CHESTNUT (CASTANEA SATIVA MILL.) SEEDLINGS UNDER FIELD CONDITIONS

F. Meotto | S. Pellegrino | G. Bounous

31. EFFECT OF CLONALLY PROPAGATED INTERSPECIFIC HYBRID CHESTNUT ROOTSTOCKS ON SHORT-TERM GRAFT COMPATIBILITY WITH FOUR CULTIVARS OF ITALIAN “MARRONE”

J.H. Craddock | G. Bassi

32. RESEARCHES ON THE ROOTSTOCK POTENTIALITY OF CHESTNUT TYPES (CASTANEA SATIVA MILL.) GROWN IN MARMARA REGION

A. Soylu | A. Eris | M. Özgür | Z. Dalkiliç

33. RESEARCHES ON STOCK-SCION COMPATIBILITY BETWEEN SOME IMPORTANT CHESTNUT CULTIVARS AND HYBRID ROOTSTOCKS

S. Ufuk | A. Soylu

34. RELATIONSHIP BETWEEN GRAFTING METHODS AND CROWN GROWTH IN CHESTNUT (CASTANEA SATIVA MILL)

A.M. Proietti Placidi

35. EFFECT OF ROOTING CONDITIONS ON SURVIVAL AND GROWTH DURING ACCLIMATIZATION OF MICROPROPAGATED CHESTNUT PLANTS (CASTANEA SATIVA X C. CRENATA)

J.C. Gonçalves | G. Diogo | M.T. Coelho | S. Amâncio

36. EFFECT OF SERIAL GRAFTING ON MICROPROPAGATION OF CHESTNUT (CASTANEA SATIVA MILL.)

A. Giovannelli | R. Giannini

37. RESEARCHES ON MICROPROPAGATION OF CHESTNUT

A. Soylu | Ü. Ertürk

38. INDUCTION OF ADVENTITIOUS SHOOTS FROM INTERNODAL LONGITUDINAL SECTIONS OF CASTANEA SATIVA MILL.

M. Mulin | M.S. Pais | R. da Costa Seabra

39. CHARACTERISTICS OF CHESTNUT COPPICES IN LIMOUSIN

J.P. Verger

40. GENETIC VARIATION AND POPULATION STRUCTURE OF THREE ENDEMIC CASTANEA SPECIES IN CHINA

P. Lang | .

41. EVALUATION OF MICROSATELLITE MARKERS FOR CHARACTERIZING CHESTNUT CULTIVARS

42. ISOZYME POLYMORPHISMS IN PORTUGUESE CHESTNUT CULTIVARS

M.J.P. Pereira | L.F.T. Castro | J.M.G. Torres-Pereira | S.P. Lorenzo

43. PRODUCTION OF PROBES FOR THE CHESTNUT GENOTYPES BY RAPD MARKERS

D. Paffetti | E. Bellini | R. Giannini

44. MOLECULAR TYPING OF ROOTSTOCK HYBRIDS (CASTANEA SATIVA X CASTANEA CRENATA) AND PORTUGUESE CASTANEA SATIVA CULTIVARS BASED ON RAPD MARKERS

C. Santana | C.M. Oliveira | T. Valdiviesso

45. THE STUDY OF CHESTNUT TREE GENETICAL RESOURCES AND THEIR PHENOTYPIC SELECTION IN ALBANIA

D. Dano

46. RESULTS OF THE FIRST CONTROLLED CROSSES IN EUROPEAN CHESTNUT IN THE SLOVAK REPUBLIC

M. Bolvansky | L. Mendel

47. PERFORMANCE AND DESCRIPTION OF THE INTRODUCED CHESTNUT CULTIVAR ‘COLOSSAL’ IN CUNEO PROVINCE, NORTHWEST ITALY

G. Bassi | J.H. Craddock

48. INTRODUCTION INTO ITALY OF EIGHT CASTANEA MOLLISSIMA CULTIVARS FROM CHINA

J.H. Craddock | G. Bassi

49. CHESTNUT BREEDING PROGRAM IN NATIONAL INSTITUTE OF FRUIT TREE SCIENCE, JAPAN

K. Kotobuki | T. Saito | Y. Kashimura | M. Shoda

50. SELECTION OF CHESTNUTS (C. SATIVA MILL.) IN SINOP VICINITY

Ü. Serdar

51. SELECTION OF CHESTNUTS (C. SATIVA MILL.) IN SAMSUN VICINITY

Ü. Serdar | A. Soylu

52. INHERITANCE OF SOME FRUIT AND FLOWER CHARACTERISTICS IN FULL – SIB PROGENIES OF CHESTNUT

M. Bolvansky | L. Mendel

53. ADAPTATION TO DROUGHT IN EUROPEAN CHESTNUT. EVIDENCES FROM A HYBRID ZONE AND FROM CONTROLLED CROSSES BETWEEN DROUGHT AND WET ADAPTED POPULATIONS

54. INK DISEASE RESISTANCE: SOME PRELIMINARY ELEMENTS FROM THE STUDY OF DIFFERENT CROSSES

M. Ramos Guedes-Lafargue | G. Salesses

55. ASSESSMENT OF RESISTANCE TO CHESTNUT INK DISEASE

56. RESISTANCE TEST FOR CHESTNUT AGAINST CRYPHONECTRIA (ENDOTHIA) PARASITICA

J. Rodriguez | C. Colinas

57. BREEDING BLIGHT-RESISTANT AMERICAN CHESTNUTS FOR REGIONAL ADAPTATION

R.C. Leffel

58. EVALUATION OF CHINESE CHESTNUT CULTIVARS FOR RESISTANCE TO CRYPHONECTRIA PARASITICA

L. Qin | X. Gao | J. Cheng | S. Liu

59. CHESTNUT RESEARCH IN CONNECTICUT: BREEDING AND BIOLOGICAL CONTROL

S.L. Anagnostakis

60. GENETIC STRUCTURE AND QUANTITATIVE TRAITS VARIATION IN F₁ FULL-SIBS PROGENIES OF CASTANEA SATIVA MILL.

61. GENETIC TRANSFORMATION OF EUROPEAN CHESTNUT (CASTANEA SATIVA MILL.) WITH GENES OF INTEREST

R. da Costa Seabra | M.S. Pais

62. CHESTNUT PESTS IN CENTRAL ITALY

S. Speranza

63. HEALTH CONDITION OF THE SWEET CHESTNUT IN CROATIA AND THE PROTECTION MEASURES IN PRACTICE

S. Novak Agbaba

64. INTEGRATED MONITORING OF CHESTNUT STANDS

T. Turchetti | G. Maresi | L. Gherardi | F. Ferretti | G.M.L. Viti

65. SOIL FACTORS, FARMER’S PRACTICES AND CHESTNUT INK DISEASE: SOME INTERACTIONS

E. Portela | J. Aranha | A. Martins | A.L. Pires

66. A METHOD FOR EVALUATING THE DEGREE OF DEFOLIATION ON CHESTNUT TREES AFFECTED BY THE INK DISEASE

M.T. Oliveira | L.M. Martins | C.G. Abreu

67. SOILS AND CLIMATIC CHARACTERISTIC OF CHESTNUT STANDS THAT DIFFER ON THE PRESENCE OF THE INK DISEASE

L.M. Martins | M.T. Oliveira | C.G. Abreu

68. SWEET CHESTNUT INCOMPATIBILITY AND MOSAICS CAUSED BY THE CHESTNUT MOSAIC VIRUS (CHMV)

J.C. Desvignes

69. BIOLOGICAL CONTROL OF CHESTNUT WEEVIL (CURCULIO ELEPHAS GYLL.; COLEOPTERA, CURCULIONIDAE) WITH THE ENTOMOPATHOGEN FUNGUS BEAUVERIA BASSIANA (BALSAMO) VUILL. (DEUTEROMYCOTINA, HYPHOMYCETES)SP1¶

B. Paparatti | S. Speranza

70. PRELIMINARY REPORT ON THE OCCURRENCE OF CRYPHONECTRIA PARASITICA (MURRILL) BARR ON DIFFERENT TREE SPECIES IN FRIULI VENEZIA-GIULIA (ITALY)

G. Frigimelica | M. Faccoli

71. THE PRODUCTION AND DISPERSAL OF ASCOSPORES OF CRYPHONECTRIA PARASITICA IN AN ORCHARD IN SOUTH-WESTERN FRANCE

L. Guérin | S. Bastien | B. Chauvin

72. CHESTNUT BLIGHT: MONITORING THE HOST RESPONSE WITH HETEROLOGOUS CDNA PROBES

R. Schafleitner | E. Wilhelm

73. CHESTNUT BLIGHT IN BOSNIA-HERZEGOVINA AND ITS CONTROL

M. Uscuplic | M. Trestic

74. STUDIES ON CHESTNUT BLIGHT (CRYPHONECTRIA PARASITICA (MURR.) BARR) IN NORTH-EAST SPAIN

C. Colinas | M. Uscuplic

75. CHESTNUT BLIGHT AND THE HYPOVIRULENCE IN THE CARPATHIAN-BASIN

L. Radócz

76. INVESTIGATIONS ON NATURAL SPREAD OF CHESTNUT BLIGHT HYPOVIRULENCE IN ITALY AND OTHER EUROPEAN COUNTRIES

T. Turchetti | G. Maresi

77. RESULTS ON BIOLOGICAL CONTROL OF CASTANEA SATIVA IN SLOVAKIA

G. Juhásová | K.A. Kulcsárová | H. Ivanová | C. Robin

78. PROBLEMS OF CHESTNUT-GROWING IN GREECE SCREENING FOR RESISTANCE OF SEVERAL CHESTNUT PROVENANCES TO CRYPHONECTRIA (ENDOTHIA) PARASITICA (MURR.) ADERSON

S.G. Xenopoulos | A. Papachatzis