Articles
STRUCTURAL AND MICROANALYTICAL STUDIES ON FROZEN-HYDRATED TISSUES FROM DIFFERENT KIWIFRUIT ORGANS
Article number
498_22
Pages
185 – 202
Language
Abstract
The analysis of ion distribution and accumulation at various time of plant development and for different physiological conditions in fruit tree organs is fundamental to understand the quali-quantitative mineral requirement in order to enhance fruit production.
Biochemical assays cannot easily provide the spatial localisation of mineral elements distribution and storage at cell-tissue level.
To overcome these problems and provide a reliable pattern of ion content and distribution, cryofixed samples maintained in their frozen-hydrated state can be analysed by Low Temperature Scanning Electron Microscopy (LTSEM) and EDX X-ray.
Biochemical assays cannot easily provide the spatial localisation of mineral elements distribution and storage at cell-tissue level.
To overcome these problems and provide a reliable pattern of ion content and distribution, cryofixed samples maintained in their frozen-hydrated state can be analysed by Low Temperature Scanning Electron Microscopy (LTSEM) and EDX X-ray.
In this article, some preliminary structural and analytical investigations performed with LTSEM and EDX X-ray microanalysis on frozen-hydrated kiwifruit leaf, peduncle and fruit samples will be presented.
Publication
Authors
C. Vitagliano, L. Sebastiani, A. Minnocci
Keywords
Actinidia deliciosa, calcium, LTSEM, Microanalysis, ion localisation, potassium
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