Articles
ROOTING OF AXILLARY SHOOTS OF CODIAEUM VARIEGATUM BLUME CV. EXCELLENT OBTAINED IN VITRO FROM DEFOLIATED SHOOT EXPLANTS
Article number
530_30
Pages
253 – 258
Language
Abstract
Shoots of Codiaeum variegatum ‘Excellent’ produced in vitro on defoliated explants were rooted on the MS medium with 1 mg l-1 indole-3-butyric acid (IBA). Almost all shoots, even the smallest one (5 mm long) rooted and doubled their length in the course of 4 weeks.
In order to stimulate more shoot elongation during in vitro rooting, we decreased the IBA concentration to 0.5 mg l-1, excluded IBA from the medium with 3 g l-1 activated charcoal (AC) or supplemented AC medium with gibberellic acid (GA3). GA3 incresed shoot length insignificantly but also decreased the number of rooted shoots.
Diluting the MS medium by half stimulated root growth when combined with 2 mg l-1 Gelrite instead of 7 g l-1 agar.
In order to stimulate more shoot elongation during in vitro rooting, we decreased the IBA concentration to 0.5 mg l-1, excluded IBA from the medium with 3 g l-1 activated charcoal (AC) or supplemented AC medium with gibberellic acid (GA3). GA3 incresed shoot length insignificantly but also decreased the number of rooted shoots.
Diluting the MS medium by half stimulated root growth when combined with 2 mg l-1 Gelrite instead of 7 g l-1 agar.
Publication
Authors
T. Orlikowska, I. Sabala, D. Kucharska
Keywords
activated charcoal, gelling agent, gibberellic acid, IBA, mineral salt concentration
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