Articles
IDENTIFICATION OF CULTIVARS AND HYBRIDS IN SYRINGA AND CUCURBITA BY DNA FINGERPRINTING
Article number
530_53
Pages
455 – 462
Language
Abstract
We have tested the applicability of AFLP analysis in varietal identification in Syringa and parentage identification after hybrid breeding in Cucurbita in a practical micropropagation situation.
Both in Syringa as well as in Cucurbita, AFLP fingerprints were highly reproducible and produced similar band patterns irrespective of the starting material being either tissue culture-derived or greenhouse material.
A single primer combination yielded enough polymorphic bands to unequivocally discriminate among 13 closely related lilac cultivars.
Following hybrid production after wide crosses between Cucurbita pepo x C. maxima lines AFLP fingerprints clearly identified the parental genomes in regenerating hybrid plants and could be used to discriminate between true hybrids and gynogenic haploids or spontaneous diploids.
Both in Syringa as well as in Cucurbita, AFLP fingerprints were highly reproducible and produced similar band patterns irrespective of the starting material being either tissue culture-derived or greenhouse material.
A single primer combination yielded enough polymorphic bands to unequivocally discriminate among 13 closely related lilac cultivars.
Following hybrid production after wide crosses between Cucurbita pepo x C. maxima lines AFLP fingerprints clearly identified the parental genomes in regenerating hybrid plants and could be used to discriminate between true hybrids and gynogenic haploids or spontaneous diploids.
Publication
Authors
M. Pfosser, H. Yemelyanava, W. Leonhardt
Keywords
Syringa spp., Cucurbita spp., AFLP, varietal identification, wide crosses, embryo rescue
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