Articles
PCR-BASED TECHNIQUES FOR THE DETECTION OF PLANT VIRUSES AND VIROIDS
Article number
530_5
Pages
61 – 67
Language
English
Abstract
Ten years after the original reports demonstrating the feasibility of the detection of plant viruses and viroids by PCR-based techniques, a large body of literature describes the development of PCR primer pairs and of very sensitive assays for essentially all groups of viral pathogens.
The tests developed show, in general, a sensitivity in great excess (up to several thousand-fold) of that reported for serological assays.
Yet, the assays reported have so far and to a very large extent been confined to laboratory scale set-ups.
Transition from this situation to the real-life, large scale application of these techniques will require further validation as well as development of automated or semi-automated pre- and post-PCR steps.
Past and current achievements as well as future perspectives are discussed.
The tests developed show, in general, a sensitivity in great excess (up to several thousand-fold) of that reported for serological assays.
Yet, the assays reported have so far and to a very large extent been confined to laboratory scale set-ups.
Transition from this situation to the real-life, large scale application of these techniques will require further validation as well as development of automated or semi-automated pre- and post-PCR steps.
Past and current achievements as well as future perspectives are discussed.
Publication
Authors
T. Candresse, M. Lanneau, F. Revers, S. Kofalvi, G. Macquaire
Keywords
detection, diagnostic, typing, sensitivity, specificity
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