Articles

THE ESTABLISHMENT OF GNOTOBIOTIC CULTURES: INVESTIGATION OF THE MYCORRHIZAL-HOST INTERACTION WITH FLAVONOIDS IN VITRO

Article number
530_41
Pages
345 – 352
Language
Abstract
The effects of plant culture medium composition and strength on Glomus mosseae (BEG 12) spore germination were monitored.
Aseptic spores were used to infect wild strawberry (Fragaria vesca), strawberry (Fragaria x ananassa) potato (Solanum tuberosum), onion (Allium cepa), tomato (Lycopersicon esculentum) and mungbean (Vigna radiata) in autotrophic culture in vitro. Three flavonoids, naringenin, hesperetin and apigenin were assessed for their effects on spore germination and root colonisation in autotrophic culture.
All three compounds significantly promoted spore germination and, all but naringenin early hyphal growth.
These effects were concentration and time dependent.
All flavonoids were observed to increase the % root length colonisation by the AMF in A. cepa and L. esculentum; only apigenin promoted colonization in S. tuberosum.

Publication
Authors
J.G. Murphy, E.M. Duffy, A.C. Cassells
Keywords
Fragaria spp., Glomus mosseae, Solanum tuberosum, Hortifoam®, micropropagation, Lycopersicon esculentum, autotrophic micropropagation, photoautotrophic culture
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