Articles
EFFECT OF IRRADIANCE LEVEL DURING IN VITRO PROPAGATION OF ARISTOLOCHIA MANCHURIENSIS
Article number
530_47
Pages
403 – 410
Language
Abstract
The ornamental plant Aristolochia manchuriensis is a liana with large heart-shaped leaves.
It is difficult to propagate by cuttings and therefore a propagation method in vitro has been developed.
The basic medium for multiplication was that of Murashige and Skoog (1962), supplemented with 6-benzylaminopurine, 0.4 mg l-1. During multiplication the following photosynthetic photon flux (PPF) levels were tested; 20, 40, 60, and 80 μmol m-2 S-1. Effects of PPF levels on multiplication stabilized after three subcultures (84 d); the PPF level 20 μmol m -2 s-1 resulted in the lowest multiplication rate (MR) per month (3.0), and 80 μmol m-2 s-1 in the highest MR per month (4.2). PPF level during multiplication also affected the subsequent rooting.
The maximum percentage of rooted shoots was attained after a PPF level of 80 μmol m -2 s-1 during multiplication.
A prolonged period on the last multiplication medium before rooting (6 weeks instead of 4 weeks) increased the multiplication rate significantly but affected subsequent rooting negatively, which dropped from 41 % to 12 %. Darkness during the first days (1 d or 7 d) on the rooting medium, improved the percentage of rooted shoots.
It was also beneficial to split the base of the shoot prior to rooting.
It is difficult to propagate by cuttings and therefore a propagation method in vitro has been developed.
The basic medium for multiplication was that of Murashige and Skoog (1962), supplemented with 6-benzylaminopurine, 0.4 mg l-1. During multiplication the following photosynthetic photon flux (PPF) levels were tested; 20, 40, 60, and 80 μmol m-2 S-1. Effects of PPF levels on multiplication stabilized after three subcultures (84 d); the PPF level 20 μmol m -2 s-1 resulted in the lowest multiplication rate (MR) per month (3.0), and 80 μmol m-2 s-1 in the highest MR per month (4.2). PPF level during multiplication also affected the subsequent rooting.
The maximum percentage of rooted shoots was attained after a PPF level of 80 μmol m -2 s-1 during multiplication.
A prolonged period on the last multiplication medium before rooting (6 weeks instead of 4 weeks) increased the multiplication rate significantly but affected subsequent rooting negatively, which dropped from 41 % to 12 %. Darkness during the first days (1 d or 7 d) on the rooting medium, improved the percentage of rooted shoots.
It was also beneficial to split the base of the shoot prior to rooting.
Publication
Authors
M. Svensson
Keywords
subculture period, darkness, wounding
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