Articles
A PRELIMINARY STUDY OF CITRUS GERMPLASM CONSERVATION AND ITS EVALUATION BY RAPD ANALYSIS
Article number
535_10
Pages
99 – 106
Language
Abstract
Existing citrus germplasm repositories have collected most of the commercial cultivars, varieties and wild species or relatives.
However, “backyard” germplasm, a link between commercial cultivars and wild species in countries with a long history of citriculture, is not well preserved and will gradually disappear.
The collection and preservation of this kind of germplasm through biotechnology is of great importance to the world citrus industry.
A program, aimed at collection, evaluation by DNA markers, and further preservation, was initiated in China in 1997. Random amplied polymorphic DNA (RAPD) was used to analyze and establish the fingerprints for more than 250 accessions of citrus.
The procedure and corresponding primers for each step were established to identify the differences and similarities of these cultivars and species.
This will improve the identification and effective preservation of backyard germplasm collected in the future.
More than 30 accessions, including some backyard types from different areas of China, were induced to produce embryogenic calli last year; in 14 of them the embryogenic calli were derived from aborted ovules of mature fruits or from the nucellar tissue of young fruits.
The first phase of this study will focus on loose-skinned citrus, since China has a long history of cultivation and wide genetic diversity in this type.
The methodology for preserving the calli and the control of somaclonal variation is being studied with the support of the National Natural Science Foundation of China (NSFC).
However, “backyard” germplasm, a link between commercial cultivars and wild species in countries with a long history of citriculture, is not well preserved and will gradually disappear.
The collection and preservation of this kind of germplasm through biotechnology is of great importance to the world citrus industry.
A program, aimed at collection, evaluation by DNA markers, and further preservation, was initiated in China in 1997. Random amplied polymorphic DNA (RAPD) was used to analyze and establish the fingerprints for more than 250 accessions of citrus.
The procedure and corresponding primers for each step were established to identify the differences and similarities of these cultivars and species.
This will improve the identification and effective preservation of backyard germplasm collected in the future.
More than 30 accessions, including some backyard types from different areas of China, were induced to produce embryogenic calli last year; in 14 of them the embryogenic calli were derived from aborted ovules of mature fruits or from the nucellar tissue of young fruits.
The first phase of this study will focus on loose-skinned citrus, since China has a long history of cultivation and wide genetic diversity in this type.
The methodology for preserving the calli and the control of somaclonal variation is being studied with the support of the National Natural Science Foundation of China (NSFC).
Authors
X.X. Deng, C.G. Hu, H. Huo, W.W. Gou, H. Yi
Keywords
citrus, germplasm, conservation, RAPD marker
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