Articles
CITRUS SOMATIC HYBRIDS REGENERATED FROM PROTOPLAST ELECTROFUSION
Article number
535_19
Pages
163 – 168
Language
Abstract
Citrus genetic improvement through conventional methods is greatly hindered by nucellar polyembryony, pollen and/or ovule sterility, and wide incompatibility.
Protoplast fusion is an alternative that can be used to circumvent these difficulties and efficiently transfer resistances to biotic and abiotic stress to commercial scion cultivars and rootstocks.
More than 20 interspecific, intergeneric, and intertribal somatic hybrids have recently been obtained in our program by electrically induced fusion.
The hybrids include those from elite scion cultivars such as Bonanza navel orange + Red blush grapefruit, Newhall navel orange + Eureka lemon, Newhall navel + Kuigan, Kinnow + Bendizao tangerine, and those from complementary rootstocks such as red tangerine + trifoliate orange, red tangerine + rough lemon, red tangerine + citrange.
Fusion between Citrus and its wild relatives such as Page tangelo + Murraya paniculata and C. sinensis + Clausena lansium (6X) regenerated allo-tetraploid and allo-hexaploid plants respectively.
By standard symmetric fusion, diploid mesophyll parent type plants (putative cybrids) from more than ten combinations were also regenerated.
To verify their hybridity, all the regenerants were identified by morphology, cytology, isozyme analysis and random amplified polymorphic DNA (RAPD) analysis.
Further identification by restriction fragment length polymorphism (RFLP) and horticultural evaluation is in progress.
Protoplast fusion is an alternative that can be used to circumvent these difficulties and efficiently transfer resistances to biotic and abiotic stress to commercial scion cultivars and rootstocks.
More than 20 interspecific, intergeneric, and intertribal somatic hybrids have recently been obtained in our program by electrically induced fusion.
The hybrids include those from elite scion cultivars such as Bonanza navel orange + Red blush grapefruit, Newhall navel orange + Eureka lemon, Newhall navel + Kuigan, Kinnow + Bendizao tangerine, and those from complementary rootstocks such as red tangerine + trifoliate orange, red tangerine + rough lemon, red tangerine + citrange.
Fusion between Citrus and its wild relatives such as Page tangelo + Murraya paniculata and C. sinensis + Clausena lansium (6X) regenerated allo-tetraploid and allo-hexaploid plants respectively.
By standard symmetric fusion, diploid mesophyll parent type plants (putative cybrids) from more than ten combinations were also regenerated.
To verify their hybridity, all the regenerants were identified by morphology, cytology, isozyme analysis and random amplified polymorphic DNA (RAPD) analysis.
Further identification by restriction fragment length polymorphism (RFLP) and horticultural evaluation is in progress.
Authors
X.X. Deng, W.W. Guo, G.H. Yu
Keywords
somatic hybridization, RAPD, genetic improvement, Aurantioideae
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