Articles
CLONING AND CHARACTERIZATION OF TWO CITRUS MITOGEN-ACTIVATED PROTEIN KINASE GENES AND EFFECTS OF ETHYLENE ON THEIR EXPRESSION PATTERNS
Article number
535_11
Pages
107 – 112
Language
Abstract
The influence of the simple gas ethylene on the activity of a Citrus myelin basic protein kinase was investigated.
Continuous ethylene treatment was found to activate a protein kinase.
This kinase also seemed to be induced by wounding.
Full-length cDNAs of two Citrus mitogen-activated protein kinase genes (CMAPK1 and CMAPK2) were isolated from a Citrus cDNA library.
Their recombinant proteins produced in bacteria were recognized by anti-human ERK antibodies, and the in-gel kinase activity assay showed that CMAPK2 recombinant protein was capable of autophosphorylation.
Northern blot analysis showed that ethylene induced both genes transcriptionally. CMAPK2 was also induced by wounding treatment, whereas CMAPK1 was not. In situ hybridization revealed that both of the MAPK genes had unusual transcript distribution patterns across the fruit abscission zone.
Continuous ethylene treatment was found to activate a protein kinase.
This kinase also seemed to be induced by wounding.
Full-length cDNAs of two Citrus mitogen-activated protein kinase genes (CMAPK1 and CMAPK2) were isolated from a Citrus cDNA library.
Their recombinant proteins produced in bacteria were recognized by anti-human ERK antibodies, and the in-gel kinase activity assay showed that CMAPK2 recombinant protein was capable of autophosphorylation.
Northern blot analysis showed that ethylene induced both genes transcriptionally. CMAPK2 was also induced by wounding treatment, whereas CMAPK1 was not. In situ hybridization revealed that both of the MAPK genes had unusual transcript distribution patterns across the fruit abscission zone.
Authors
G.Y. Zhong, R. Goren, J. Riov, D. Holland
Keywords
mitogen-activated protein kinase, ethylene, wounding
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