Articles
GENETIC POLYMORPHISM OF SWEET ORANGE (Citrus sinensis [L.] Osbeck) VARIETIES EVALUATED BY RANDOM AMPLIFIED POLYMORPHIC DNA
Article number
535_5
Pages
51 – 54
Language
Abstract
Genomic DNA was extracted from sweet orange (Citrus sinensis [L.] Osbeck) varieties in the germplasm collection of the Centro de Citricultura Sylvio Moreira, Instituto Agronomico, Cordeirópolis, SP, Brazil.
Representatives of each sweet orange group were assayed using random amplified polymorphic DNA (RAPD) to characterize and detect polymorphisms among the varieties.
In spite of phenotypic differences, no polymorphisms were detected.
We concluded that RAPD analysis is not applicable in differentiation of sweet orange varieties.
More sensitive methods of analysis should be used.
Representatives of each sweet orange group were assayed using random amplified polymorphic DNA (RAPD) to characterize and detect polymorphisms among the varieties.
In spite of phenotypic differences, no polymorphisms were detected.
We concluded that RAPD analysis is not applicable in differentiation of sweet orange varieties.
More sensitive methods of analysis should be used.
Authors
M.L.P. Natividade Targon, M.A. Machado, H.D. Coletta Filho, M. Cristofani
Keywords
sweet orange, RAPD, fingerprint, molecular marker
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