Articles
CLONING AND CHARACTERISATION OF PPI-PHOSPHOFRUCTOKINASE FROM CITRUS FRUIT
Article number
535_12
Pages
113 – 118
Language
Abstract
As part of a study of sugar metabolism in citrus fruit, a full-length cDNA encoding for the two subunits of the PPi-dependent phosphofructokinase (PFP
PFP
) were cloned from grapefruit juice sac cells.
The clones share over 85% homology with PFPs of other plants.
Expression of the genes was studied during orange fruit development.
Both genes exhibit the highest level of expression in the flower.
A different pattern is observed in fruits: PFP is barely detected in young fruits of 20–30 mm, and is induced in 35–40 mm fruits, whereas PFP is constitutively expressed in fruits of 20–40 mm.
These results suggest that the ratio of PFP/PFP changes at various stages of fruit development.
PFP) were cloned from grapefruit juice sac cells.The clones share over 85% homology with PFPs of other plants.
Expression of the genes was studied during orange fruit development.
Both genes exhibit the highest level of expression in the flower.
A different pattern is observed in fruits: PFP
is barely detected in young fruits of 20–30 mm, and is induced in 35–40 mm fruits, whereas PFP is constitutively expressed in fruits of 20–40 mm.These results suggest that the ratio of PFP
/PFP changes at various stages of fruit development.
Authors
R. Kapri, E. Dahan, A. Sadka, U. Zehavi, R. Goren
Keywords
Citrus paradisi, Citrus sinensis, fructose-6-phosphate, fructose-1,6-bisphosphate, fructose-2,6-bisphosphate, PPi-dependent phosphofructokinase
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