Articles
ISOLATION AND IDENTIFICATION OF ISOQUERCITRIN FROM EXTRACTS OBTAINED FROM LEAVES OF MORUS ALBA (L.) AND MORUS NIGRA (L.)
Article number
597_13
Pages
105 – 114
Language
English
Abstract
The isolation of isoquercitrin from the leaves of Morus alba L. and Morus nigra L. is part of a greater plan of research.
It consists in linking the flavonoids isolated from the leaves and bark of Morus sp. with biologically-active metallic ions.
The aim is to obtain some semisynthetic compounds with the potential to become new pharmaceutical products.
Isoquercitrin was isolated by column chromatography and purified by recrystallisation using a solvent combination (MeOH/H2O). The purified compound was subject to analysis by spectroscopic (UV/VIS, IR, 1H-NMR) and electrochemical methods (polarography).
By comparison of chromatographic parameters (Rf=0,55; tR=8,00 min), spectral data (
max Band I = 361 nm, Band II = 258, 2666 sh), polarographical parameters (E1/2 = – 1,604 V) and melting point (186-187°), the isolated substance was shown to be identical to the standard substance (Rf = 0,55; tR = 7,80 min, max Band I = 359 nm, Band II = 258, 266 sh; E1/2 = -1,604 V; pt° = 188-189). Thus, the isolated flavonglycoside is isoquercitrin, in an advanced grade of purity.
IR and 1H-NMR spectra also confirmed this result.
Thus the separation of the flavonoid complex is a laborious method, but suitable choice of solvents/method leads to satisfactory results.
It consists in linking the flavonoids isolated from the leaves and bark of Morus sp. with biologically-active metallic ions.
The aim is to obtain some semisynthetic compounds with the potential to become new pharmaceutical products.
Isoquercitrin was isolated by column chromatography and purified by recrystallisation using a solvent combination (MeOH/H2O). The purified compound was subject to analysis by spectroscopic (UV/VIS, IR, 1H-NMR) and electrochemical methods (polarography).
By comparison of chromatographic parameters (Rf=0,55; tR=8,00 min), spectral data (
max Band I = 361 nm, Band II = 258, 2666 sh), polarographical parameters (E1/2 = – 1,604 V) and melting point (186-187°), the isolated substance was shown to be identical to the standard substance (Rf = 0,55; tR = 7,80 min, max Band I = 359 nm, Band II = 258, 266 sh; E1/2 = -1,604 V; pt° = 188-189). Thus, the isolated flavonglycoside is isoquercitrin, in an advanced grade of purity.IR and 1H-NMR spectra also confirmed this result.
Thus the separation of the flavonoid complex is a laborious method, but suitable choice of solvents/method leads to satisfactory results.
Authors
D. Muntean, C. Csedö
Keywords
Morus alba, Morus nigra, flavonglycoside, isoquercitrin
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