Articles
ANTIFUNGAL ACTIVITY OF SOME MEDICINAL PLANT EXTRACTS AGAINST CANDIDA ALBICANS AND CRYPTOCOCCUS NEOFORMANS
Article number
597_31
Pages
217 – 221
Language
English
Abstract
The ethanol extracts of clove (Eugenia caryophyllus Bullock & Harrison) and sweet flag (Acorus calamus Linn.) were investigated for their antifungal activity in comparison with eugenol and amphotericin B (AmB) by using the National Committee for Clinical Laboratory Standards (NCCLs) M27-P broth microdilution method.
Two medicinal plant extracts, eugenol and amphotericin B were used to determine their minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) against 28 clinical isolates of Candida albicans and 25 clinical isolates of Cryptococcus neoformans. The MICs of clove, sweet flag, eugenol and AmB against C. albicans were 17.41±8.64 mg/ml, 28.8±16.32 mg/ml, 12.16±4.53 mg/ml and 0.23±0.1 µg/ml respectively.
The MFCs were 67.5±15.39 mg/ml, >75 mg/ml, 15.4±6.47 mg/ml and 0.47±0.21 µg/ml respectively.
The same extracts and antifungal drugs which were tested against C. albicans were also tested against C. neoformans.
The MICs were 2.43±0.95 mg/ml, 3.02±1.97 mg/ml, 6.28±3.4 mg/ml and 0.28±0.15 µg/ml, respectively.
The MFCs were 22.22±12.71 mg/ml, 30.82±27.11 mg/ml, 10.06±4.9 mg/ml and 0.51±0.25 µg/ml respectively.
The results showed that C. albicans was significantly (p<0.01) more susceptible to the extract of clove than sweet flag, whereas C. neoformans was significantly susceptible to the clove extract (p>0.05). Moreover, the extract of clove showed significantly (p<0.01) more potent inhibitory activity against C. neoformans than eugenol, while it showed significantly (p<0.01) less inhibitory activity against C. albicans than eugenol.
AmB, the drug of choice for invasive infection treatment, remains as one of the most effective antifungal drugs.
These data indicate that the extracts of clove and sweet flag were potential fungistatic agents against yeasts, whereas AmB and eugenol showed fungicidal effects.
Two medicinal plant extracts, eugenol and amphotericin B were used to determine their minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) against 28 clinical isolates of Candida albicans and 25 clinical isolates of Cryptococcus neoformans. The MICs of clove, sweet flag, eugenol and AmB against C. albicans were 17.41±8.64 mg/ml, 28.8±16.32 mg/ml, 12.16±4.53 mg/ml and 0.23±0.1 µg/ml respectively.
The MFCs were 67.5±15.39 mg/ml, >75 mg/ml, 15.4±6.47 mg/ml and 0.47±0.21 µg/ml respectively.
The same extracts and antifungal drugs which were tested against C. albicans were also tested against C. neoformans.
The MICs were 2.43±0.95 mg/ml, 3.02±1.97 mg/ml, 6.28±3.4 mg/ml and 0.28±0.15 µg/ml, respectively.
The MFCs were 22.22±12.71 mg/ml, 30.82±27.11 mg/ml, 10.06±4.9 mg/ml and 0.51±0.25 µg/ml respectively.
The results showed that C. albicans was significantly (p<0.01) more susceptible to the extract of clove than sweet flag, whereas C. neoformans was significantly susceptible to the clove extract (p>0.05). Moreover, the extract of clove showed significantly (p<0.01) more potent inhibitory activity against C. neoformans than eugenol, while it showed significantly (p<0.01) less inhibitory activity against C. albicans than eugenol.
AmB, the drug of choice for invasive infection treatment, remains as one of the most effective antifungal drugs.
These data indicate that the extracts of clove and sweet flag were potential fungistatic agents against yeasts, whereas AmB and eugenol showed fungicidal effects.
Authors
S. Thirach, K. Tragoolpua, S. Punjaisee, C. Khamwan, C. Jatisatienr, N. Kunyanone
Keywords
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