Articles
EFFECT OF MACROELEMENTS ON THE GROWTH AND LOBELINE PRODUCTION OF LOBELIA INFLATE L. HAIRY ROOT CULTURES
Article number
597_35
Pages
245 – 251
Language
English
Abstract
Lobelia inflata L. contains piperidine alkaloids.
The main alkaloid is the pharmacologically active lobeline.
We have studied the effect of macroelements (Mg2+, Na+ and Ca2+) on the growth and alkaloid production of hairy root cultures of L. inflata (clone 8009/f4). Macroelements influenced characteristically the linear growth and biomass formation of hairy roots.
The greatest biomass formed in B5 media containing 1000 mg/l MgSO4. It was found that the highest amount of lobeline (39 µg/g, dry weight) was reached at 75 mg/l CaCl2. The results suggest that the optimal nutrient mixture for the best lobeline production was the basal B5 medium (containing 150 mg/l CaCl2, 150 mg/l NaH2PO4) supplemented with 1000 mg/l MgSO4.
The main alkaloid is the pharmacologically active lobeline.
We have studied the effect of macroelements (Mg2+, Na+ and Ca2+) on the growth and alkaloid production of hairy root cultures of L. inflata (clone 8009/f4). Macroelements influenced characteristically the linear growth and biomass formation of hairy roots.
The greatest biomass formed in B5 media containing 1000 mg/l MgSO4. It was found that the highest amount of lobeline (39 µg/g, dry weight) was reached at 75 mg/l CaCl2. The results suggest that the optimal nutrient mixture for the best lobeline production was the basal B5 medium (containing 150 mg/l CaCl2, 150 mg/l NaH2PO4) supplemented with 1000 mg/l MgSO4.
Authors
I. Bálványos, E. Szóke, L. Kursinszki
Keywords
hairy root,Lobelia inflate, lobeline, macroelements
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