Articles
IDENTIFICATION AND MEASUREMENT OF ENDOGENOUS FORMALDEHYDE IN DATURA INNOXIA MILL. CALLUS TISSUES BY OPLC, HPLC AND MALDI MS TECHNIQUES
Article number
597_38
Pages
265 – 270
Language
English
Abstract
The purpose of the present study was to determine the presence of measurable amount of endogenous formaldehyde (HCHO) in callus tissue cultures of Datura innoxia Mill. applying different analytical methods.
The effect of HCHO deprivation with dimedone – as abiotic stress – was also investigated on the growth and endogenous HCHO level in D. innoxia callus cultures.
In our work, HCHO in dimedone adduct form (formaldemethone), has been isolated and characterised by overpressured layer chromatography (OPLC), high pressure liquid chromatography (HPLC), and matrix assisted laser desorption / ionization mass spectrometry (MALDI MS). Endogenous HCHO deprivation with dimedone affected the cell proliferation in D. innoxia callus tissues as well.
The effect of HCHO deprivation with dimedone – as abiotic stress – was also investigated on the growth and endogenous HCHO level in D. innoxia callus cultures.
In our work, HCHO in dimedone adduct form (formaldemethone), has been isolated and characterised by overpressured layer chromatography (OPLC), high pressure liquid chromatography (HPLC), and matrix assisted laser desorption / ionization mass spectrometry (MALDI MS). Endogenous HCHO deprivation with dimedone affected the cell proliferation in D. innoxia callus tissues as well.
Authors
I. László, E. Szóke, E. Tyihák, Z. Németh, L. Albert
Keywords
abiotic stress, Datura innoxia Mill., dimedone, formaldemethone
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