Articles
CULTIVATION OF LOBELIA INFLATA L. HAIRY ROOT CULTURE IN BIOREACTOR
Article number
597_36
Pages
253 – 256
Language
English
Abstract
We have studied the biomass and alkaloid production of genetically transformed hairy root cultures of Lobelia inflate L.. The hairy root clone 8009/h7 transformed with Agrobacterium rhizogenes strain R1601 was cultivated on B5 liquid media containing 3% sucrose.
The bioactive compounds were analysed by HPLC, the total alkaloid content was determined by spectrophotometry in the hairy roots and in the liquid medium.
The bioreactor cultures produced an increased biomass and quantity of total alkaloids, but lobeline was not measurable in the tissues and in the medium. 1.7 mg/g total alkaloid content was found in the hairy root tissues.
The total alkaloid production in the culture medium (14.3 mg) was higher than in the tissues (10.46 mg). Polyacetylenes were detected both in the tissues and in the liquid medium.
The bioactive compounds were analysed by HPLC, the total alkaloid content was determined by spectrophotometry in the hairy roots and in the liquid medium.
The bioreactor cultures produced an increased biomass and quantity of total alkaloids, but lobeline was not measurable in the tissues and in the medium. 1.7 mg/g total alkaloid content was found in the hairy root tissues.
The total alkaloid production in the culture medium (14.3 mg) was higher than in the tissues (10.46 mg). Polyacetylenes were detected both in the tissues and in the liquid medium.
Authors
P. Bányai, I. Bálványos, L. Kursinszki, E. Szöke
Keywords
bioreactor, hairy root, Lobelia inflate, piperidine alkaloids, lobeline
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