Articles
A COMPARATIVE STUDY OF DAPHNE VIRUSES X, S AND Y
Article number
59_16
Pages
95 – 96
Language
Abstract
Three distinct anisometric viruses, daphne virus X (DVX), daphne virus S (DVS) and daphne virus Y (DVY), were identified from Daphne spp. in New Zealand.
All three have filamentous particles: DVX are 499 nm long with indistinct cross banding; DVS 716 nm long with longitudinal grooving; and DVY 733 nm long with distinct cross banding.
The sedimentation coefficients of DVX, DVS and DVY are 110S, 174S and 149S respectively.
DVX has an A260/280 ratio of 1.22 and DVS, 1.35. Serologically, DVX is distantly related to white clover mosaic virus and DVS to carnation latent virus, but DVY was unrelated to nine potyviruses tested.
However DVY resembles potyviruses in several ways, including transmission by sap and aphids (nonpersistent), relative instability in crude sap, limited host range, and association with cylindrical inclusions in DVY-infected tissues.
The similarity of DVX to other potyviruses includes ready sap transmission to a wide range of plants, stability in crude sap and frequent occurence of spiral and lateral virus aggregates in DVX-infected tissues.
DVS has not been transmitted by either sap or vector but its restricted host range and latency in many daphne plants, relative instability in crude sap, and production of cellular inclusions characteristic of the carlaviruses, confirm its affinity with the carlavirus group.
All three have filamentous particles: DVX are 499 nm long with indistinct cross banding; DVS 716 nm long with longitudinal grooving; and DVY 733 nm long with distinct cross banding.
The sedimentation coefficients of DVX, DVS and DVY are 110S, 174S and 149S respectively.
DVX has an A260/280 ratio of 1.22 and DVS, 1.35. Serologically, DVX is distantly related to white clover mosaic virus and DVS to carnation latent virus, but DVY was unrelated to nine potyviruses tested.
However DVY resembles potyviruses in several ways, including transmission by sap and aphids (nonpersistent), relative instability in crude sap, limited host range, and association with cylindrical inclusions in DVY-infected tissues.
The similarity of DVX to other potyviruses includes ready sap transmission to a wide range of plants, stability in crude sap and frequent occurence of spiral and lateral virus aggregates in DVX-infected tissues.
DVS has not been transmitted by either sap or vector but its restricted host range and latency in many daphne plants, relative instability in crude sap, and production of cellular inclusions characteristic of the carlaviruses, confirm its affinity with the carlavirus group.
Authors
K.S. Milne, R.L. Forster
Keywords
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