Articles
ASSESSMENT OF ANTIOXIDANT ACTIVITY OF PLANT EXTRACTS BY DIFFERENT METHODS
Article number
677_13
Pages
99 – 107
Language
English
Abstract
The aim of this study was to assess the radical scavenging and antioxidant activity (AA) of several aromatic and medicinal plant extracts by different methods: measurement of free radical scavenging activity (RSA) [reactions with 1,1-diphenyl-2-picrylhydrazyl radical (DPPH• and 2,2-azinobis(3-ethylbenzo-thiazo-line-6-sulfonate) (ABTS+)]; assessment of the influence of extracts on the enzyme xanthine oxidase (XO), their effects in carotene-methyl linoleate cooxidation system and Fe3+ reducing ability; evaluation of the effect on oilseed oil stabilization (peroxide value, binding of oxygen, UV absorption). It was demonstrated that the use of different methods is very advisable.
This approach opens the possibility for more comprehensive characterization of extract role in the complicated oxidation processes.
For instance, the comparison of RSA obtained in DPPH and ABTS test systems did not show linear relationship.
Rosemary, lemon balm and peril extracts possessed well-expressed RSA with DPPH, but they were not so active in ABTS test system; savory extract had insignificant RSA with DPPH, but in ABTS test system was more active.
The relationship between RSA and extract AA in oil was also rather complicated.
Some extracts were quite good radical scavengers (similar to rosemary and sage), whereas their effect on oil stabilization was low or medium.
XO is one of the most important enzymes producing O2–. in vivo, therefore, its inhibitors in some cases could be beneficial.
The most effective inhibitors of XO were extracts from sage, oregano, Roman chamomile, tansy, rosemary, costmary, lemon balm, thyme, birch, hyssop, nettle and peril.
Plant extracts also were able to reduce Fe3+.
This approach opens the possibility for more comprehensive characterization of extract role in the complicated oxidation processes.
For instance, the comparison of RSA obtained in DPPH and ABTS test systems did not show linear relationship.
Rosemary, lemon balm and peril extracts possessed well-expressed RSA with DPPH, but they were not so active in ABTS test system; savory extract had insignificant RSA with DPPH, but in ABTS test system was more active.
The relationship between RSA and extract AA in oil was also rather complicated.
Some extracts were quite good radical scavengers (similar to rosemary and sage), whereas their effect on oil stabilization was low or medium.
XO is one of the most important enzymes producing O2–. in vivo, therefore, its inhibitors in some cases could be beneficial.
The most effective inhibitors of XO were extracts from sage, oregano, Roman chamomile, tansy, rosemary, costmary, lemon balm, thyme, birch, hyssop, nettle and peril.
Plant extracts also were able to reduce Fe3+.
Publication
Authors
P.R. Venskutonis, Gruzdienè, D. Tirzite, G. Tirzitis
Keywords
radical scavenging, xanthine oxidase inhibition, reduction of ferric ions, rapeseed oil oxidation
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